Fig 1: Immunoblot data for eIF2, AMPK, and rpS6 at days 1, 3, 7, and 35 of whole-brain homogenates. Phosphorylated (p) and total (t) proteins were detected and expressed as p/t ratios to reflect activation of proteins. Ponceau staining was used to normalize for total protein loading. (A) At day 3, mice fed a High Fat SAAR diet had significantly lower eIF2 activation compared to control. (B) Phosphorylated AMPK was undetectable in whole-brain homogenate; only t-AMPK is analyzed. At day 3, both the Regular Fat SAAR and High Fat SAAR groups had greater t-AMPK content than control. (C) Phosphorylated AMPK was detectable in pre-frontal cortex. Due to limited pre-frontal cortex tissue, only p/t ratios for day 1 and day 35 were assessed and no differences were observed. (D) rpS6 activation tended to be lower in the Regular Fat SAAR group at day 1, however, at day 35 was significantly greater than control. Data are presented as mean ± SD (n = 3/group). eIF2, eukaryotic initiation factor 2; AMPK; AMP-activated protein kinase; rpS6, ribosomal protein S6. *p < 0.05, **p < 0.01, # 0.05 < p < 0.10. SAAR diets are compared to their respective SAA sufficient control diets.