Fig 1: Lysosomal dysfunction in the ventral thalamus and CA3 hippocampal region of aged GrnR493X/R493X mice. Representative hippocampal/thalamic tilescans highlighting autofluorescent lipofuscin (a, green channel), Lamp1 (b), and DppII (c) accumulation in 18 month old GrnR493X/R493X mice (scale bar, 500 µm). d, e Representative Lamp1 and DppII immunofluorescence images of hippocampal CA3 and thalamic VPM/VPL regions in 18 month old Grn+/+ and GrnR493X/R493X brain sections (scale bar, 20 µm). f, g Total Lamp1-positive and DppII-positive area in CA3 (i) and VPM/VPL (ii) images were quantified and normalized to the total number of cells (DAPI, blue). For immunofluorescence staining n = 10 mice were used per sex/genotype (except male GrnR493X/R493X n = 8); values are shown as mean ± SEM; **p < 0.01, ***p < 0.0001, Student’s t-test