HAND1 Antibody AF3168 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHAND1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF3168
NCBI Gene AliasesThing1, bHLHa27, eHand, Hxt
ApplicationsWestern Blots,Flow Cytometry,Immunohistochemistry,Western Blot,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry
IsotypeIgG
HostGoat
ReactivityHuman,Mouse
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetHAND1
ImmunogenE. coli-derived recombinant human HAND1, Met1-Gln215, Accession # O96004
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
Antibody TypePrimary Antibodies
ClonalityPolyclonal
NCBI Full Gene Nameheart and neural crest derivatives expressed 1
Quantity100 ug (also 25 ug)
PurityAntigen Affinity-purified
ConcentrationLYOPH

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human HAND1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HAND2 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) HAND1, partially mediated through ape-specific LTR binding, is essential for human extra-embryonic mesenchyme derivation from iPSCsCell ReportsApril 22, 2025Zukai Liu, Yuliana Tan, William F. Flynn, Lili Sun, Ponthip Pratumkaew, Juliana Alcoforado Alcoforado Diniz, Nelio A.J. Oliveira, Justin A. McDonough, William C. Skarnes, Paul RobsonRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies anti-ANKS1A,BETHYL,Cat #: A303-050A; RRID: AB_10893960 anti-HAND1,R&D Systems,Cat #: AF3168; RRID: AB_2115853 anti-GAPDH,Cell Signaling Technology,Cat #: 2118S; RRID: AB_561053 HRP-conjugated Affinipure Donkey Anti-Goat IgG(H + L

(2) EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotencyScience advancesApril 11, 2025Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, Iwasaki S, Takahashi K.

(3) HAND1 level controls the specification of multipotent cardiac and extraembryonic progenitors from human pluripotent stem cellsThe EMBO JournalMarch 31, 2025Adam T Lynch, Naomi Phillips, Megan Douglas, Marta Dorgnach, I-Hsuan Lin, Antony D Adamson, Zoulfia Darieva, Jessica Whittle, Neil A Hanley, Nicoletta Bobola, et al.Lab,Addgene ID 19780 Antibodies,Antibodies,Antibodies Polyclonal Goat anti-HAND1 (1 µg/mL),R & D Systems,#AF3168 Monoclonal Mouse anti-PDGFRα-BV421 (1:50),BD Biosciences,#526799 Monoclonal Mouse anti-CD235a-APC

(4) Combinatorial interpretation of BMP and WNT controls the decision between primitive streak and extraembryonic fates in human pluripotent stem cellsCell SystemsMay 15, 2024Elena Camacho-Aguilar, Sumin T. Yoon, Miguel A. Ortiz-Salazar, Siqi Du, M. Cecilia Guerra, Aryeh Warmflash,BD Biosciences,611203 Nanog,Mouse,1:400,BD Biosciences,560482 Nanog,Goat,1:200,R&D Systems,AF1997 Hand1,Goat,1:200,R&D Systems,AF3168 Gata3,Rabbit,1:100,ThermoFisher,PA1-101 Lef1,Rabbit,1:200,Cell Signalling Technologies,2230s Tfap2α,Mouse,1:50,DHSB,3B5 Eomes,Rabbit,1:200,Abcam,Ab23345 phosphoSmad1,Rabbit,1:100,Cell Signalling

(5) Spatial Single Cell Analysis of Proteins in 2D Human Gastruloids Using Iterative ImmunofluorescenceCurrent ProtocolsApril 20, 2024Emily Freeburne, Seth Teague, Hina Khan, Bolin Li, Siyuan Ding, Bohan Chen, Adam Helms, Idse Heemskerk,R&D Systems,MAB6166,1/200 FOXA2,R&D Systems,AF2400,1/500 GATA3,SantaCruz Biotechnology,sc-268,1/50 HAND1,R&D Systems,AF3168,1/200 ISL1,DSHB,39.4D5,1/200 LEF1,Cell Signaling Tech,C12A5,1/100 MIXL1,ABclonal,A17223,1/150 NANOG,R&D Systems

(6) Characterization of an induced pluripotent stem cell line NCHi011-A from a 23-year-old female with Alagille Syndrome harboring a heterozygous JAG1 pathogenic variantStem cell researchOctober 1, 2023Stanberry I, Cunningham D, Ye S, Alonzo M, Zhao MT, Garg V, Lilly B.

(7) Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 geneStem cell researchSeptember 1, 2023Cunningham D, Stanberry I, Ye S, Alonzo M, Zhao MT, Garg V, Lilly B.

(8) Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cellsBiology openAugust 15, 2023Gattiglio M, Protzek M, Schröter C.Fisher Scientific, eBioMLC-51), anti-T/BRA 1:200 (R&D Systems, AF2085), anti-HAND1 1:200 (R&D Systems, AF3168), anti-TBX6 1:200 (R&D Systems, AF4744) and anti-CDX2 1:250 (BioGenex, MU392A-5UC). Secondary antibodies

(9) Origin and segregation of the human germlineLife science allianceAugust 1, 2023Castillo-Venzor A, Penfold CA, Morgan MD, Tang WW, Kobayashi T, Wong FC, Bergmann S, Slatery E, Boroviak TE, Marioni JC, et al.Santa Cruz Biotechnology), anti-OCT4 (611203; BD Biosciences), anti-TFAP2A (sc-12726; Santa Cruz Biotechnology), anti-HAND1 (AF3168; R&D Systems), anti-GATA3 (ab199428; Abcam), and anti-NANOG (AF1997; R&D). Secondary antibodies used

(10) Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlayCell Rep MethodsJune 26, 2023Overeem AW, Chang YW, Moustakas I, Roelse CM, Hillenius S, Helm TV, Schrier VFV, Gonçalves MAFV, Mei H, Freund C, et al.

(11) The time integral of BMP signaling determines fate in a stem cell model for early human developmentbioRxiv : the preprint server for biologyApril 10, 2023Teague S, Primavera G, Chen B, Freeburne E, Khan H, Jo K, Johnson C, Heemskerk I.#,Vendor ISL1,Mouse,1:200,39.4D5,DSHB SOX2,Rabbit,1:200,3579S,Cell Signaling Technology NANOG,Goat,1:100,AF1997,R&D Systems HAND1,Goat,1:200,AF3168,R&D Systems GATA3,Rabbit,1:800,5852S,Cell Signaling Technology TFAP2C,Mouse,1:150,SC-12762,Santa Cruz Biotechnology

(12) CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndromeStem cell researchMarch 1, 2023Akdaş EY, Turan S, Guhathakurta D, Ekici A, Salar S, Lie DC, Winner B, Fejtova A.

(13) Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)Stem cell researchDecember 1, 2022Lin H, Ye SQ, Xu ZH, Penaloza JS, Aljuhani M, Vetter T, Zhao MT, McBride KL.

(14) Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation modelCell reportsJune 21, 2022Ohgushi M, Taniyama N, Vandenbon A, Eiraku M.

(15) A stress-reduced passaging technique improves the viability of human pluripotent cellsCell Rep MethodsFebruary 28, 2022Takahashi K, Okubo C, Nakamura M, Iwasaki M, Kawahara Y, Tabata T, Miyamoto Y, Woltjen K, Yamanaka S.anti-SOX17,R&D Systems,"Cat# AF1924, RRID:AB_355060" Goat polyclonal anti-HAND1,R&D Systems,"Cat# AF3168, RRID:AB_2115853" Rabbit polyclonal anti-PAX6,BioLegend,"Cat# 901301, RRID:AB_2565003" Mouse monoclonal

(16) Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosisStem cell researchDecember 1, 2021Mehta A, Lu P, Taylor BV, Charlesworth J, Cook AL, Burdon KP, Hewitt AW, Young KM.

(17) Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart DevelopmentInternational journal of molecular sciencesSeptember 11, 2021Zheng M, Erhardt S, Ai D, Wang J.

(18) Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloidsPLoS biologyOctober 1, 2019Chhabra S, Liu L, Goh R, Kong X, Warmflash A.CA",Cat#MU392A GATA3(1:100),"Thermo Fisher Scientific, Waltham, MA",Cat#PA1-101 HAND1(1:200),R&D Systems,Cat#AF3168 ISL1(1:50),"Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA",Cat#39.4D5 NANOG

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Human HAND1 Antibody

HAND1 Antibody

HAND1 Antibody

HAND1 Antibody

HAND1 Antibody

HAND1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (18)

Reviews

HAND1 Antibody from R&D Systems, a Bio-Techne Brand

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