(±)-Blebbistatin, inhibitor of myosin II ab120425 from Abcam

Product Specs
Item(±)-Blebbistatin, inhibitor of myosin II
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab120425
Quantity5 mg, 25 mg
Purity0.99
TypeInhibitor
Molecule NameBlebbistatin

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Website: http://www.abcam.com

(1) Clinically Relevant Doses of Remimazolam Modulate Cardiac Electrophysiology: Late Repolarization Prolongation and Increased Conduction Dispersion With Preserved QTcCardiol Res PractApril 13, 2026Zijun Wang, Ying Cao, Gao Su, Yanyan Feng, Rongfeng Yang, Xue Bai, Hong GaoLangendorff system. Following stabilization, contractions were suppressed by 10 μmol/L blebbistatin (Abcam, ab120425). To enhance dye uptake, 20% Pluronic F‐127 (Invitrogen, P3000MP) was perfused prior to dye loading.

(2) Cell loss disrupts mechanical homeostasis to drive retinal pigment epithelium ageing-like phenotype in vitroNat CommunApril 8, 2026Teodora Piskova, Aleksandra N. Kozyrina, Giedrė Astrauskaitė, Mohamed Elsafi Mabrouk, Sebastian Schepl, Stacy Lok Sze Yam, Ragul Ravithas, Wolfgang Wagner, Massimo Vassalli, Jacopo Di Russowith DMSO as a carrier control. Myosin II inhibition was performed using 5 µM blebbistatin (Abcam, ab120425) for 1 h before nanoindentation and for 30 min before POS addition in blebbistatin-containing medium

(3) Programmable acoustofluidic engineering for creating gradient biomaterialsSci AdvDecember 19, 2025Yujing Lu, Ye He, Jianping Xia, Mingyuan Liu, Joseph Rich, Ke Jin, Ruoyu Zhong, Kaichun Yang, Jiao Qian, Ying Chen, et al.contractility inhibition study Cells were treated by two actomyosin-inhibiting agents, blebbistatin (Abcam, ab120425; 50 μM) and Y-27632 (Abcam, ab120129; 10 μM) for 24 hours. After that, the samples were used for cyto-immunofluorescence

(4) M2 macrophage co-culture overrides viscoelastic hydrogel mechanics to promote IL-6-dependent fibroblast activationCell BiomaterialsMay 27, 2025Leilani R. Astrab, Mackenzie L. Skelton, Steven R. Caliarimolecule,Actin polymerization,5 μM,Abcam (ab144290) (±)-Blebbistatin,Small molecule,Myosin II,50 μM,Abcam (ab120425) Y-27632 dihydrochloride,Small molecule,Rho kinase,10 μM,Abcam (ab120129) List of inhibitors used

(5) Influence of Myosin Regulatory Light Chain and Myosin Light Chain Kinase on the Physiological Function of Inner Ear Hair CellsJournal of the Association for Research in OtolaryngologyApril 16, 2025Ryohei Oya, Kwang Min Woo, Brian Fabella, R. G. Alonso, Paloma Bravo, A. J. Hudspeth(Agilent); VECTASHIELD Antifade Mounting Medium (Vector Laboratories, Newark, CA, USA); blebbistatin (ab120425; Abcam); protease XXIV (Sigma-Aldrich, St. Louis, MO, USA); PeriAcryl90 (GluStitch Inc., Delta, BC,

(6) Membrane mechanics dictate axonal pearls-on-a-string morphology and functionNature neuroscienceJanuary 1, 2025Griswold JM, Bonilla-Quintana M, Pepper R, Lee CT, Raychaudhuri S, Ma S, Gan Q, Syed S, Zhu C, Bell M, et al.1 h before the experiment. Stocks were used within 1 month. Blebbistatin (±)-Blebbistatin (Abcam, ab120425) was dissolved in DMSO to a stock concentration of 10 mM, added to the cells to a final concentration

(7) Capsule release surgery temporarily reduces contracture in a rat elbow model of arthrofibrosisJournal of Orthopaedic ResearchJanuary 1, 2025Austin J. Scholp, Jordan A. Jensen, Timothy P. Fowler, Emily Petersen, Douglas Fredericks, Aliasger K. Salem, Dongrim Seol, Mitchell Coleman, Spencer P. Lake, James A. Martin, et al.fascial and skin layers was again performed with absorbable sutures. 2.4 Blebbistatin Blebbistatin (ab120425 Abcam) was diluted to 1 mg/mL in a vehicle of 0.9% saline with 6.7% dimethyl sulfoxide (DMSO) and 25%

(8) Direct M2 macrophage co-culture overrides viscoelastic hydrogel mechanics to promote fibroblast activationbioRxivOctober 19, 2024Leilani R. Astrab, Mackenzie L. Skelton, Steven R. Caliarimolecule,Actin polymerization,5 μM,Abcam (ab144290) (±)-Blebbistatin,Small molecule,Myosin II,50 μM,Abcam (ab120425) Y-27632 dihydrochloride,Small molecule,Rho kinase,10 μM,Abcam (ab120129) List of inhibitors used

(9) Brain-to-heart cholinergic synapse-calcium signaling mediates ischemic stroke-induced atrial fibrillationTheranosticsOctober 7, 2024Yingran Liang, Gongxin Wang, Siwen Fan, Junyi Zhang, Shuang He, Guixiang Pan, Guoliang Hao, Yan Zhupurchased from Chengdu Lemeitian Pharmaceutical Technology Co., Ltd (Chengdu, China). Blebbistatin (ab120425) and Rhod-2AM (ab142780) were obtained from Abcam Plc (Cambridge, United Kingdom). F127 (P3000MP) was

(10) Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventionsNature communicationsSeptember 12, 2024Yang P, Zhu L, Wang S, Gong J, Selvaraj JN, Ye L, Chen H, Zhang Y, Wang G, Song W, et al.glucose at 37 °C and 10 mL/min flow rate. After 15 minutes of stabilization, 1 mg/mL blebbistatin (Abcam, ab120425) was perfused into the heart to eliminate motion artifacts. Intracellular calcium changes were measured

(11) Actomyosin-II protects axons from degeneration induced by mild mechanical stressJournal of Cell BiologyAugust 5, 2024Xiaorong Pan, Yiqing Hu, Gaowei Lei, Yaxuan Wei, Jie Li, Tongshu Luan, Yunfan Zhang, Yuanyuan Chu, Yu Feng, Wenrong Zhan, et al.listed suppliers: SiR-Actin (#CY-SC001; Cytoskeleton); Oligomycin (#11342; Cayman); Blebbistatin (#ab120425; Abcam); PNB (#24171; Cayman); ML-7 (#11801; Cayman); Calyculin A (#9902S; Cell Signaling); BAPTA-AM

(12) Lumen expansion is initially driven by apical actin polymerization followed by osmotic pressure in a human epiblast modelCell Stem CellMay 2, 2024Dhiraj Indana, Andrei Zakharov, Youngbin Lim, Alexander R. Dunn, Nidhi Bhutani, Vivek B. Shenoy, Ovijit Chaudhuriculture (for plots labelled continuous inhibition). The inhibitors used were Blebbistatin (10 μM; Abcam ab120425, myosin II inhibitor), ML-7 (10 μM; Tocris Bioscience 4310, myosin light chain kinase inhibitor), ML-141

(13) A comprehensive protocol combining in vivo and ex vivo electrophysiological experiments in an arrhythmogenic animal modelAmerican Journal of Physiology-Heart and Circulatory PhysiologyJanuary 1, 2024Mathias Nyman, Tomas O. Stølen, Anne Berit Johnsen, Karin Garten, Francis L. Burton, Godfrey L. Smith, Jan Pål Loennechenmmol/L 2,3-butanedione monoxime (BDM; Sigma-Aldrich, Cat. No. B0753) and 10 µmol/L blebbistatin (Abcam, ab120425). Leave the heart to stabilize for 10 min before loading of the voltage-sensitive dye (VSD) (FluoVolt

(14) Chronological adhesive cardiac patch for synchronous mechanophysiological monitoring and electrocoupling therapyNature CommunicationsOctober 6, 2023Chaojie Yu, Mingyue Shi, Shaoshuai He, Mengmeng Yao, Hong Sun, Zhiwei Yue, Yuwei Qiu, Baijun Liu, Lei Liang, Zhongming Zhao, et al.the MI (n = 5) and CAHP (n = 5) groups were perfused with excitation-contraction uncoupling reagent (ab120425, Abcam) via the Langendorff system to gradually stop beating but maintain electrophysiological activity.

(15) Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusionNat CommunAugust 24, 2023Chao Jiang, Hong-Yu Luo, Xinpeng Xu, Shuo-Xing Dou, Wei Li, Dongshi Guan, Fangfu Ye, Xiaosong Chen, Ming Guo, Peng-Ye Wang, et al.FITC-dextran (D7136) and 70-kD rhodamine B-dextran (D1841) were from Molecular Probes. Blebbistatin (ab120425) and Calyculin A (ab141784) were from Abcam. Latrunculin A and Jasplakinolide were from Sigma-Aldrich.

(16) Force-Bioreactor for Assessing Pharmacological Therapies for Mechanobiological TargetsFrontiers in Bioengineering and BiotechnologyJuly 19, 2022Austin J. Scholp, Jordan Jensen, Sathivel Chinnathambi, Keerthi Atluri, Alyssa Mendenhall, Timothy Fowler, Aliasger K. Salem, James A. Martin, Edward A. Sandersystem was quantified and compared to the response of the system to the addition of 50 µM blebbistatin (ab120425, Abcam, Waltham, MA) in 0.1% dimethyl sulfoxide (DMSO). Three samples for each condition were tested

(17) Protocol to record and quantify the intracellular pH in contracting cardiomyocytesSTAR ProtocolsJune 17, 2022Yankun Lyu, Valeriy Timofeyev, James Overton, Phung N. Thai, Ebenezer N. Yamoah, Nipavan Chiamvimonvat, Xiao-Dong ZhangM3671 "2,3-Butanedione Monoxime (BDM)","Sigma-Aldrich, USA",Cat# B0753 (±)-Blebbistatin,"Abcam, USA",Cat# Ab120425 (-)-Isoproterenol hydrochloride (ISO),Sigma-Aldrich,Cat# I6504 5-(N-Ethyl-N-isopropyl) amiloride (EIPA),Sigma-Aldrich,Cat#

(18) Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signalingCell death and differentiationMarch 1, 2022Hu S, Ren S, Cai Y, Liu J, Han Y, Zhao Y, Yang J, Zhou X, Wang X.Selleck Chemicals (TX, USA). AT56 was from Cayman (13160, MI, USA) and Blebbistatin was bought from Abcam (ab120425, MA, USA). Tunicamycin (ab120296, Abcam) and PNGase F (P0704S, New England Biolabs, MA, USA) were purchased,

(19) Beat-to-beat dynamic regulation of intracellular pH in cardiomyocytesiScienceJanuary 21, 2022Yankun Lyu, Phung N. Thai, Lu Ren, Valeriy Timofeyev, Zhong Jian, Seojin Park, Kenneth S. Ginsburg, James Overton, Julie Bossuyt, Donald M. Bers, et al.AM,ThermoFisher Scientific,P35373 Carboxy SNARF®-1 AM,ThermoFisher Scientific,C1272 (±)-Blebbistatin,Abcam,Ab120425 "2,3-Butanedione monoxime","Sigma-Aldrich, USA",B0753 (-)-Isoproterenol hydrochloride,Sigma-Aldrich,I6504 5-(N-Ethyl-N-isopropyl) amiloride

(20) Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytesSTAR ProtocolsDecember 1, 2021Xiao-Dong Zhang, Maria Cristina Perez Flores, Valeriy Timofeyev, Ebenezer N. Yamoah, Nipavan ChiamvimonvatCorning,025052 "2,3-Butanedione monoxime (BDM)","Sigma-Aldrich, USA",B0753 Blebbistatin,"Abcam, USA.",Ab120425 Salicylic acid,"Sigma-Aldrich, USA",W398500 Collagenase type II,"Worthington, USA",LS004176 Ketamine hydrochloride

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(±)-Blebbistatin, inhibitor of myosin II from Abcam

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(±)-Blebbistatin, inhibitor of myosin II from Abcam

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