Fig 1: ZNF185 is essential for PKA-induced phosphorylation of RhoGDIα.a RhoGDIα knockdown induces RhoA activation. RhoA activity in HUVECs was measured using a rhotekin pull-down assay. Representative images are shown (n = 3). b–d. RhoGDIα knockdown induces stress fiber formation in a confluent HUVECs monolayer. b Immunofluorescence staining of actin (green) and VE-cadherin (magenta). Representative images are shown (n = 3). Scale bars, 25 µm. c Intensity of stress fibers at the yellow lines in Fig. 5b was quantified. d The bar graph shows total amount of stress fiber intensity in Fig. 5c. Two-sided Student’s t test, *p < 0.05 (n = 3). e ZNF185 knockdown suppressed RhoGDIα phosphorylation. Western blots and densitometry analysis of phospho-RhoGDI. Representative blots are shown. Two-sided Student’s t test, *p < 0.05 (n = 3). Data are presented as the mean ± standard error. Ctrl control, Fsk forskolin.