Western Blot was performed using Anti-CD55 Polyclonal Antibody (Product # PA5-21532) and a 70 kDa band corresponding to Complement decay-accelerating factor was observed across positive cell line (HeLa); and not across negative cell lines (IMR-32 and SH-SY5Y). Whole cell extracts (30 µg lysate) of HeLa (Lane 1), IMR-32 (Lane 2) and SH-SY5Y (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).