Western blot was performed using Anti-RALA Rabbit Polyclonal Antibody (Product # PA5-22333) and a 26 kDa band corresponding to RALA was observed across cell line and tissues tested along with an uncharacterized band (*) at ~110 kDa. Membrane enriched cell extracts (30 µg lysate) of NTERA-2 (Lane 1), tissue extracts of Mouse brain (Lane 2), and Rat brain (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).