Fig 1: CP204L promotes lysosomal clustering and blocks VPS39 integration into the HOPS complex. (A) WSL cells stably expressing VPS39-GFP were analyzed by confocal fluorescence microscopy after transfection with a CP204L expression plasmid or infection with ASFV. Cells were stained for the lysosomal marker anti-LAMP1. (B) Quantification of the colocalization of VPS39 with lysosomes labeled by LAMP1. Pearson’s coefficient (mean ± standard error of the mean [SEM]) from 25 cells in each group. (C) Quantification of the number and area of lysosomal and late endosomal aggregates per cell (n = 25 cells in each group). ****, P < 0.0001. (D) VPS11 staining shows a loss of colocalization between VPS39 and VPS11 in virus-infected cells. Arrows indicate virus factories. Bars, 10 μm.