Western blot analysis was performed on membrane enriched extracts of SH-SY5Y (Lane 1), HeLa (Lane 2), Neuro-2a (Lane 3), HEK 293 (Lane 4), A-431 (Lane 5) and NIH/3T3 (Lane 6). The blot was probed with Anti-PAK2 antibody (Product # PA5-20254, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27034, 0.25 µg/mL, 1:4000 dilution). A 60 kDa band corresponding to PAK2 was observed across the cell lines tested.