Fig 1: 661W photoreceptor cells were incubated with Poly(dA:dT) (5 µg/ml) ± WD (50 µM) or DMSO (0.01%) for 8 h prior to analysis. (a) Relative mRNA expression of inflammasome components in 661W photoreceptor cells, compared to the reference gene Actb. n = 3 per group; n represents the average of three experimental repeats. (b) Representative immunocytochemistry images of AIM2 (green) and CASP11 (red) taken from treated 661W photoreceptor cells, counterstained with DAPI (blue) to visualise the nucleus. Scale bar: 50 µm. (c) The relative protein expression and release of inflammatory cytokine IL18 was quantified through ELISA. The results were calculated by interpolating the absorbance values against a standard curve of known IL18 concentrations. n = 3 per group; n represents the average of three experimental repeats. (d) Cell viability of 661W photoreceptor cells was determined by YO-PRO-1 uptake after 8 h of treatment; n = 6 per group. One-way ANOVA with Tukey’s post-hoc test. * p < 0.05 and *** p < 0.001: relative to the control. # p < 0.05, ## p < 0.01, ### p < 0.001: relative to Poly(dA:dT). Error bars shown as mean ± SEM.