Immunofluorescent analysis of Ubiquilin-1 in paraformaldehyde-fixed A549 cells using a Ubiquilin-1 polyclonal antibody (Product # PA5-22174) at a 1:200 dilution.

Western blot analysis was performed on whole cell extract (30 µg lysate) of SH-SY5Y (Lane 1), U-87 mg (Lane 2), RAW 264.7 (Lane 3), A431 (Lane 4), HeLa (Lane 5), PC-3 (Lane 6), A549 (Lane 7) and tissue extract of Mouse Brain (Lane 8). The blot was probed with Anti-Ubiquilin 1 Polyclonal Antibody (Product # PA5-22174, 1:3,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 63 kDa band corresponding to Ubiquilin 1 was detected in cell lines tested where as mouse origin cell line or tissue shows extremely weak signal, indicating human specificity.