GAD67 Antibody PA5-21397 from Thermo Fisher Scientific

Product Specs
ItemGAD67 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-21397
FormatPBS with 20% glycerol and 0.025% Proclin 300; pH 7
ClonalityPolyclonal
NCBI Full Gene Nameglutamate decarboxylase 1
ApplicationsWB, ICC, IHC, IHC-fr, IHC-p
ReactivityHu, Ms, Rt
Concentration0.67 mg/mL
ImmunogenRecombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary
PurityAntigen affinity chromatography
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles
Antibody TypePrimary
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
NCBI Gene AliasesGAD, Gad67, EP10, DEE89, Gad-1, SCP, CPSQ1
HostRabbit
IsotypeIgG
TargetGAD67
Conjugate/TagUnconjugated
Quantity100 µL

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(1) Human microglia in brain assembloids display region-specific diversity and respond to hyperexcitable neurons carrying SCN2A mutationScience AdvancesFebruary 20, 2026Jiaxiang Wu, Xiaoling Chen, Jingliang Zhang, Kyle Wettschurack, Morgan Robinson, Weihao Li, Yuanrui Zhao, Ye-Eun Yoo, Brody A. Deming, Yue Shu, et al.(Thermo Fisher Scientific, PA1-10005), anti-NeuN (Thermo Fisher Scientific, 702022), anti-GAD67 (Thermo Fisher Scientific, PA5-21397), anti-FOXA2 (Thermo Fisher Scientific, 701698), and anti-OTX2 (Thermo Fisher Scientific, MA5-15854),

(2) Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress‐Induced Depression in Male MiceAdvanced ScienceFebruary 1, 2026Jinyu Zhang, Binbin Zhao, Min Jia, Yan Zhao, Ye Lu, Wenyu Xi, Ziyu Zhu, Xiaojuan Gong, Qingyan Ma, Yuan Gao, et al.Cell Signaling Technology), GFAP (GB11096, Servicebio), IBA‐1 (GB12105, Servicebio), CaMKII (11533‐1‐AP, Proteintech), GAD67 (PA5‐21397, Invitrogen), c‐Fos (OB‐PGP080, Oasis biogarm), GPX4 (ab125066, Abcam), and PCBP1 (14523‐1‐AP, Proteintech).

(3) Human microglia in brain assembloids display region- specific diversity and respond to hyperexcitable neurons carrying SCN2A mutation: Microglial diversity and response in assembloidsbioRxivJune 4, 2025Jiaxiang Wu, Xiaoling Chen, Jingliang Zhang, Kyle Wettschurack, Morgan Robinson, Weihao Li, Yuanrui Zhao, Ye-Eun Yoo, Brody A. Deming, Akila D. Abeyaratna, et al.anti-MAP2 (Thermo Fisher, PA1–10005), anti-NeuN (Thermo Fisher, 702022), anti-GAD67 (Thermo Fisher, PA5–21397), anti-FOXA2 (Thermo Fisher, 701698), and anti-OTX2 (Thermo Fisher, MA5–15854). Confocal images were

(4) Parallel Gene Expression Changes in Ventral Midbrain Dopamine and GABA Neurons during Normal AgingeNeuroMay 13, 2025Ana Luiza Drumond-Bock, Harris E. Blankenship, Kevin D. Pham, Kelsey A. Carter, Willard M. Freeman, Michael J. Beckstead(1:1,000)",Millipore,Catalog #MAB369/RRID: AB_2190413 Antibody: Rabbit anti-GAD67 (1:100),Thermo Fisher Scientific,Catalog #PA5-21397/RRID: AB_11153284 Antibody: Donkey anti-goat Alexa Fluor 488 conjugate (1:500),Jackson ImmunoResearch Laboratories

(5) Type-I-interferon-responsive microglia shape cortical development and behaviorCellApril 11, 2024Escoubas CC, Dorman LC, Nguyen PT, Lagares-Linares C, Nakajo H, Anderson SR, Barron JJ, Wade SD, Cuevas B, Vainchtein ID, et al.anti-Somatostatin 1:500,Thermo Fisher,Cat#MA516987; RRID:AB_2538460 Rabbit anti-GAD67 1:500,Thermo Fisher,Cat#PA521397; RRID:AB_11153284 Goat anti-rabbit AF488 1:500,Thermo Fisher,Cat#A-11034; RRID:AB_2576217 Goat anti-rat

(6) A novel role for phospholamban in the thalamic reticular nucleusScientific reportsMarch 16, 2024Klocke B, Britzolaki A, Saurine J, Ott H, Krone K, Bahamonde K, Thelen C, Tzimas C, Sanoudou D, Kranias EG, et al.included mouse monoclonal PLN (Invitrogen #MA3-922; 1:100), rabbit polyclonal anti-GAD67 (Invitrogen #PA5-21397; 1:250), rabbit polyclonal anti-SERCA2 (Abcam #ab150435; 1:500), and rabbit polyclonal anti-GFAP (Abcam

(7) Genetic Ablation of Dentate Hilar Somatostatin-Positive GABAergic Interneurons is Sufficient to Induce Cognitive ImpairmentMolecular neurobiologyFebruary 1, 2024Nagarajan R, Lyu J, Kambali M, Wang M, Courtney CD, Christian-Hinman CA, Rudolph U.Invitrogen, cat # PA5-82678, 1:1000), anti-glutamic acid decarboxylase 67 (GAD-67, Invitrogen, cat # PA5-21397, 1:500) and anti-Iba-1 on microglia (Iba-1, Invitrogen, cat # MA5-36257, 1:500). After washing with

(8) Localized APP expression results in progressive network dysfunction by disorganizing spike timingNeuronJanuary 3, 2024Viana da Silva S, Haberl MG, Gaur K, Patel R, Narayan G, Ledakis M, Fu ML, de Castro Vieira M, Koo EH, Leutgeb JK, et al.times with 1x PBS and incubated overnight at 4°C with primary antibodies for GAD67 (1:500, Invitrogen PA5–21397) and NeuN (1:2000, Sigma-Aldrich ABN91) dissolved in a carrier solution containing 8% NGS and 0.3% Triton-X

(9) Selective inhibition of somatostatin-positive dentate hilar interneurons induces age-related cellular changes and cognitive dysfunctionPNAS NexusMay 1, 2023Lyu J, Nagarajan R, Kambali M, Wang M, Rudolph U.Invitrogen, cat # PA5-82678, 1:1,000), antiglutamic acid decarboxylase 67 (GAD-67, Invitrogen, cat # PA5-21397, 1:500), anti-Iba1 on microglia (Iba1, Invitrogen, cat # MA5-36257, 1:500), and antineuronal nuclei

(10) Differential regulation of nucleus accumbens glutamate and GABA in obesity-prone and obesity-resistant ratsJournal of neurochemistryFebruary 1, 2023Vollbrecht PJ, Nesbitt KM, Addis VM, Boulnemour KM, Micheli DA, Smith KB, Sandoval DA, Kennedy RT, Ferrario CR.synthetase (1:1000, rabbit, Millipore, catalog no. G2781), GAD-67 (1:1000, rabbit, Invitrogen, catalog no. PA5–21397), and GLT-1 (1:10,000, guinea pig, Millipore, catalog no. AB1783) diluted in TBS. Secondary antibodies

(11) Screening Biophysical Sensors and Neurite Outgrowth Actuators in Human Induced-Pluripotent-Stem-Cell-Derived NeuronsCellsAugust 9, 2022Pai VP, Cooper BG, Levin M.(SERT) 1:500 (abcam-ab272912), and rabbit anti-glutamate decarboxylase 67 (GAD67) 1:500 (Thermo Fisher-PA5-21397). The secondary antibody used was goat anti-rabbit Alexa 488 conjugated 1:1000 (Thermo Fisher-A-11070).

(12) The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the MouseFrontiers in molecular neuroscienceJanuary 1, 2022Wu JPH, Yeung J, Rahimi-Balaei M, Wu SR, Zoghbi H, Goldowitz D.Proteintech, 21215-1 AP), rabbit anti-Brn2 (1:1,000; Abcam, Ab94977), rabbit anti-GAD67 (1:500; ThermoFisher, PA5-21397), rabbit anti-Irx3 (1:8,000; a gift from Tom Jessell, Columbia University), rabbit anti-Pax6 (1:200;

(13) The effects of preventative cannabidiol in a male neuregulin 1 mouse model of schizophreniaFrontiers in cellular neuroscienceJanuary 1, 2022Visini G, Brown S, Weston-Green K, Shannon Weickert C, Chesworth R, Karl T.expression of the following protein markers: GAD67 polyclonal antibody (1:2, 000, Thermofisher Scientific [PA5-21397]), AMPA subunit GluA1 [1:10,000, Abcam (ab31232)], NMDAR subunit NR1 [Abcam (ab17345)], and actin housekeeper

(14) ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activityTranslational psychiatryJune 29, 2021Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B.anti-synaptophysin (1:500 for blotting; Dako; M7315), rabbit anti-GAD67 (1:500 for blotting; Thermos Fisher; PA5-21397), rabbit anti-actin (1:1000 for blotting; Santa Cruz; sc-1616-R), Flag-beads (for co-IP; Sigma; A2220),

(15) Assessment of the acquisition of executive function during the transition from adolescence to adulthood in male and female miceDevelopmental cognitive neuroscienceDecember 1, 2017Shepard R, Beckett E, Coutellier L.2014). Antibodies against parvalbumin (PV – ABCam #ab11427; 1:500), GAD67 (ThermoFisher Scientific #PA5-21397; 1:5000) and tyrosine hydroxylase (TH – Novus Biologicals #NB300-109; 1:1000) were used for PFC samples,

(16) Overexpression of Glutamate Decarboxylase in Mesenchymal Stem Cells Enhances Their Immunosuppressive Properties and Increases GABA and Nitric Oxide LevelsPloS oneJanuary 1, 2016Urrutia M, Fernández S, González M, Vilches R, Rojas P, Vásquez M, Kurte M, Vega-Letter AM, Carrión F, Figueroa F, et al.

(17) Region-specific effects of repeated ketamine administration on the presynaptic GABAergic neurochemistry in rat brainNeurochemistry internationalDecember 1, 2015Boczek T, Lisek M, Ferenc B, Wiktorska M, Ivchevska I, Zylinska L.

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GAD67 Antibody from Thermo Fisher Scientific

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GAD67 Antibody from Thermo Fisher Scientific

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