Western Blot analysis of CAS was performed by separating 30 µg of various whole cell lysates by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CAS Polyclonal Antibody (Product # PA5-21468) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. NIH-3T3, B. JC, C. BCL-1, D. C2C12.