Nephrin Antibody PA5-20330 from Thermo Fisher Scientific

Product Specs
ItemNephrin Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-20330
FormatPBS with 0.02% sodium azide
ClonalityPolyclonal
NCBI Full Gene Namenephrosis 1, nephrin, NPHS1 adhesion molecule, nephrin
ImmunogenA 14 amino acid peptide from near the carboxy terminus of human Nephrin
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage4°C
Storage TemperatureMaintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
NCBI Gene AliasesCNF, NPHN, NephrinB, nephrin
IsotypeIgG
ReactivityHu, Ms, Rt
ApplicationsWB, IHC-p
HostRabbit
TargetNephrin
Conjugate/TagUnconjugated
Quantity100 µg
Concentration1 mg/mL

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) A natural nephroprotective adjuvant for cancer chemotherapy: Rosmarinic acid disrupts IL-17 A-Ferroptosis coupling in Ifosfamide-induced renal injuryMed OncolMarch 8, 2026Büşra Süzen Celbek, Hasan Şimşek, Nurhan Akaras, Özge Kandemir, Hamit Emre Kızıl, Hüseyin Mutlu, Fatih Mehmet Kandemirmolecule-1 (KIM-1) (Cat. No. PA1632; Boster Biological Technology, Pleasanton, CA, USA), Nephrin (Cat. No. PA5-20330; Thermo Fisher Scientific, Waltham, MA, USA), and Caspase-3 (Cat. No. SC-56053; Santa Cruz Biotechnology,

(2) Biomimetic fractal topography enhances podocyte maturation in vitroNat CommunDecember 15, 2025Chuan Liu, Praful Aggarwal, Karl T. Wagner, Shira Landau, Teng Cui, Xin Song, Laleh Shamaei, Naimeh Rafatian, Yimu Zhao, Sonia Rodriguez-Ramirez, et al.incubated with primary antibodies, anti-podocin (Abcam, ab50339, 1:200 dilution), anti-nephrin (Invitrogen, PA5-20330, 1:500 dilution), anti-nestin (Invitrogen, MA1-110, 1:500 dilution), or anti-YAP1 (Invitrogen, PA1-46189,

(3) CRB2 depletion induces YAP signaling and disrupts mechanosensing in podocytesAmerican Journal of Physiology-Renal PhysiologyApril 1, 2025Yingyu Sun, Nils M. Kronenberg, Sidharth K. Sethi, Surjya N. Dash, Maria E. Kovalik, Benjamin Sempowski, Shelby Strickland, Rupesh Raina, C. John Sperati, Xuefei Tian, et al.primary antibodies at 4°C overnight: nephrin rabbit polyclonal antibody (1:200, Thermo Fisher Scientific, PA5–20330, RRID:AB_11153994), synaptopodin mouse monoclonal antibody (1:200, Progen Biotechnik, 61094, RRID:AB_1543006),

(4) CRB2 Depletion Induces YAP Signaling and Disrupts Mechanosensing in PodocytesbioRxiv : the preprint server for biologyOctober 26, 2024Sun Y, Kronenberg NM, Sethi SK, Dash SN, Kovalik ME, Sempowski B, Strickland S, Raina R, Sperati CJ, Tian X, et al.primary antibodies at 4°C overnight: nephrin rabbit polyclonal antibody (1:200, Thermo Fisher Scientific, PA5–20330), synaptopodin mouse monoclonal antibody (1:200, Progen Biotechnik, 61094), FAK1mouse monoclonal antibody

(5) Degradation of Ubiquitin-Editing Enzyme A20 following Autophagy Activation Promotes RNF168 Nuclear Translocation and NF-κB Activation in Lupus NephritisJournal of innate immunityJanuary 1, 2023Zou L, Sun L, Hua R, Wu Y, Sun L, Chen T.(1:50, ab89901, Abcam, Cambridge, UK) expressed by podocytes, mature podocyte markers nephrin (1:100, PA5-20330, Thermo Fisher Scientific Inc., Waltham, MA, USA) and nestin (1:20, 14-5843-82, Thermo Fisher Scientific)

(6) CCl4-nefrotoksisitesinde L-karnitin’in etkileri: Glomeruler nefrin ve HIF-1alfa ekspresyonlarının immunohistokimyasal değerlendirilmesiCukurova Medical JournalJune 30, 2020Derya KARABULUT, Emel ÖZTÜRK, Ali Tuğrul AKİN, Ayça LEKESİZCAN, Hacı Murat ÜNSAL, Tuğçe Merve ÖZYAZGAN, Meryem SAYAN2. Immunohistochemistry Immunohistochemistry was performed with avidinbiotin-peroxidase method to determine nephrin (PA5-20330, Invitrogen, USA) and HIF-1α (NB100479, Novus Biologicals, USA), immunoreactivity in kidney tissue.

(7) A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrierNature communicationsAugust 13, 2019Petrosyan A, Cravedi P, Villani V, Angeletti A, Manrique J, Renieri A, De Filippo RE, Perin L, Da Sacco S.(Rhodamine),Vector,RL-1022,IF 1:100 NPHS1 (FITC),LifeSpan Biosciences,LS-C370063,IF 1:100 Nephrin (NPHS1),Invitrogen,PA5-20330,WB: 1:1000 WT1,Abcam,ab15249,IF 1:50 "Col IV α1,2",Abcam,ab6311,IF 1:50 Col IV α3,Shigei Research

(8) A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunctionEMBO molecular medicineMay 1, 2019Lin CL, Hsu YC, Huang YT, Shih YH, Wang CJ, Chiang WC, Chang PJ.membrane (Bio‐Rad) and subjected to immunoblotting with primary antibodies. Antibodies against nephrin (PA5‐20330; Thermo Fisher Scientific; 1:2,000 dilution), KDM6A (sc‐514859; Santa Cruz; 1:100 dilution), WT‐1 (sc7385;

(9) Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor CellsStem cells internationalJanuary 1, 2019Nguyen L, Spitzhorn LS, Adjaye J.antibody,Specificity,Dilution,Company,Cat. No. CD31,Mouse,1 : 300,R&D,BBA7 NPHS1,Rabbit,1 : 200,Invitrogen,PA5-20330 NPHS2,Rabbit,1 : 400,Proteintech,20384-1-AP PAX8,Rabbit,1 : 200,Cell Signaling,59019 PDGFR-β,Rabbit,1 : 100

(10) WNT/β-catenin signaling plays a crucial role in myoblast fusion through regulation of nephrin expression during developmentDevelopment (Cambridge, England)November 27, 2018Suzuki A, Minamide R, Iwata J.

(11) Podocyte injury elicits loss and recovery of cellular forcesScience advancesJune 1, 2018Haley KE, Kronenberg NM, Liehm P, Elshani M, Bell C, Harrison DJ, Gather MC, Reynolds PA.overnight at 4°C in primary antibody: nephrin rabbit polyclonal antibody (1:200) (Thermo Fisher Scientific, PA5-20330), CD2AP rabbit polyclonal antibody (1:200) (Sigma-Aldrich, HPA003326), podocin mouse polyclonal antibody

(12) Role of γ-adducin in actin cytoskeleton rearrangements in podocyte pathophysiologyAmerican journal of physiology. Renal physiologyJanuary 1, 0001Gao W, Liu Y, Fan L, Zheng B, Jefferson JR, Wang S, Zhang H, Fang X, Nguyen BV, Zhu T, et al.

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Nephrin Antibody

Nephrin Antibody from Thermo Fisher Scientific

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Nephrin Antibody from Thermo Fisher Scientific

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