PD-L1 Antibody PA5-20343 from Thermo Fisher Scientific

Product Specs
ItemPD-L1 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-20343
Quantity100 µg
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate/TagUnconjugated
ReactivityHuman, Mouse, Rat
TargetPD-L1
HostRabbit
NCBI Gene AliasesPdcd1l1, hPD-L1, B7h1, Pdl1, PD-L1, RGD1566211, PDCD1L1, B7-H, A530045L16Rik, B7H1, Pdcd1lg1, PDCD1LG1, PDL1
FormatPBS with 0.05% sodium azide
ClonalityPolyclonal
NCBI Full Gene NameCD274 molecule, CD274 antigen
Concentration1 mg/mL
ImmunogenA 17 amino acid peptide from near the center of human PDL-1
IsotypeIgG
Storage TemperatureMaintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
PurityAntigen affinity chromatography
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Prognostic value of PD-L1 expression on tumor-infiltrating immune cells and neutrophil-to-lymphocyte ratio in patients with biliary tract cancerFrontiers in ImmunologyFebruary 9, 2026Shang Chen, Guizhong Huang, Zehui Yao, Xiaojun Lin, Jianzhong CaoXL flow cytometer (Coulter, USA). IHC was conducted using the PD-L1 Polyclonal Antibody (Catalog No. PA5-20343, Thermo-Fisher Scientific, RRID: AB_11153819, diluted 1:100). The procedure was carried out using the

(2) NG25 Enhances Anti-Tumor Immunity in KRAS-Mutant Colorectal CancerOncoTargets and TherapyJanuary 8, 2026Qi Xiang, Zhigang Mao, Qizhao Ma, Mi Su, Deng Tang, Siqi Lan, Yufei Tang, Ji Zhang, Yufang Wangsc-166562) and p-TAK1 (Cat. No. sc-4531) from Santa Cruz Biotechnology (Dallas, TX, USA); PD-L1 (Cat. No. PA5-20343) from Thermo Fisher Scientific (Waltham, MA, USA); β-actin (Cat. No. A2228) from Sigma-Aldrich (St.

(3) Sennoside A Modulates the Ferroptosis and Immune Evasion of Oral Squamous Cell Carcinoma Cells Through Inhibiting the NF-κB PathwayIntegrative Cancer TherapiesSeptember 17, 2025Jiaying Huo, Jianhua Qi, Qixuan RenAB_3094570), a rabbit polyclonal antibody against PD-L1 (1:1000 dilution, Thermo Fisher Scientific, Cat# PA5-20343, RRID: AB_11153819), a rabbit polyclonal antibody against NF-κB (1:1000 dilution, Abcam, Cat# ab16502,

(4) Programmable immunoprobiotics orchestrate antitumor immune response with Pin1 inhibition for pancreatic cancer treatmentProceedings of the National Academy of SciencesAugust 26, 2025Sichen Yuan, Xicheng Yang, Alexa M. Bremmer, Yixin Wang, Sherry Li, Yu Chen, Yawen You, Quanyin HuIgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 (A-11005), PD-L1 Polyclonal Antibody (PA5-20343), were purchased from ThermoFisher Scientific. HRP Anti-beta Actin antibody (ab49900) was purchased

(5) N6-methyladenosine modification of MEF2A weakens cetuximab sensitivity in colorectal cancer via PD-L1/SOX12 axisCell Death DiscovJuly 1, 2025Cao Gao, Jiajia He, Jiemin Zhao, Xuefeng Ni, Yanjie Xuused to perform RNA FISH assay. For immunofluorescence, cells were incubated in anti-PD-L1 antibody (PA5-20343, Invitrogen, 1:100) at 4 °C overnight. Afterwards, cells were incubated in Alexa Fluor 488 secondary

(6) The influence of H. pylori infection in HER2-positive gastric cancer cell lines: insights from Wnt/β-catenin pathwayFrontiers in ImmunologyJune 26, 2025Valli De Re, Mariateresa Casarotto, Giulia Brisotto, Stefania Zanussi, Mariangela De Zorzi, Ombretta Repetto, Elena Muraro, Paola Spessotto, Paolo Baldo, Vito Racanelli, et al.Cell Signalling Technology), p53 (DO-1) (1:1000; #sc-126, Santa Cruz Biotechnology), PD-L1 (1:500; #PA520343, Thermo Scientific), PD-L2 (D7U8C) (1:500; #82723, Cell Signalling Technology), Snail (C15D3) (1:1000;

(7) Mechanism of LINC01018/miR-182-5p/Rab27B in the immune escape through PD-L1-mediated CD8+ T cell suppression in gliomaBiol DirectMay 21, 2025Su Hu, Guoshuo Chen, Aiping Luo, Hailin Zhao, Dan Li, Biao Peng, Jike Du, Dongdong Luoincubated with the primary antibodies as follows: Rab27B (1:1000, ab317264, Abcam), PD-L1 (1:1000, PA5-20343, Invitrogen), E-Cadherin (1:1000, ab231303, Abcam), N-Cadherin (1:5000, ab76011, Abcam), Vimentin (1:3000,

(8) LGALS3BP antibody-drug conjugate enhances tumor-infiltrating lymphocytes and synergizes with immunotherapy to restrain neuroblastoma growthJournal of translational medicineApril 11, 2025Cela I, Capone E, Pece A, Lovato G, Simeone P, Colasante M, Lamolinara A, Piro A, Iezzi M, Lanuti P, et al.X-100 and labelled for 30 min at 4 °C with primary antibody anti-PD-L1 (rabbit polyclonal; 1:100, #PA5-20343; Invitrogen). Then cells were labelled for 30 min at 4 °C in the dark with AlexaFluor-488 conjugated

(9) Macrophages are activated toward phagocytic lymphoma cell clearance by pentose phosphate pathway inhibitionCell reports. MedicineDecember 17, 2024Beielstein AC, Izquierdo E, Blakemore S, Nickel N, Michalik M, Chawan S, Brinker R, Bartel HH, Vorholt D, Albert L, et al.,Cell Signaling Technology,Cat#84651; RRID:AB_2800041 Rabbit polyclonal anti-PD-L1,Thermo Fisher Scientific,Cat#PA5-20343; RRID:AB_11153819 Mouse monoclonal anti-phosphogluconate dehydrogenase (G-2) (6PGD),"Santa Cruz Biotechnology, INC

(10) Serpin B9 is Highly Expressed in Lung Adenocarcinoma and is Associated with Progression-Free SurvivalJournal of Inflammation ResearchNovember 14, 2024Yue Fang, Yi Yue, Sensen Hao, Ying Zhang, Nan Liu, Shengling Wang, Yan Li, Hongzhi Wangmin, once). The specific antibody of anti-Serpin B9 (14,910-1-AP, Proteintech, China) and anti-PDL1 (PA5-20343, Invitrogen, USA) was then added dropwise(1:200) to the tissue at the centre of the section and incubated

(11) Enhancing the Efficacy of Breast Cancer Immunotherapy Using a Smac-Armed Oncolytic VirusCancersSeptember 24, 2024Tang S, Lyles KV, Wang Y, Fan D, Luo M.the Dako EnVision FLEX system as previously described [21]. IHC expression of PD-L1 (Thermo Fisher # PA5-20343), Ki-67 (Thermo Fisher # MA5-14520), or Caspase 3 (Thermo Fisher # 19677-1-AP) was performed on lung

(12) Progestogen-driven B7-H4 contributes to onco-fetal immune toleranceCellAugust 22, 2024Jiali Yu, Yijian Yan, Shasha Li, Ying Xu, Abhijit Parolia, Syed Rizvi, Weichao Wang, Yiwen Zhai, Rongxin Xiao, Xiong Li, et al.RRID: AB_443686 Anti-B7-H4,Abcam,Cat#ab209242; RRID: AB_2801513 Anti-human PD-L1,Thermo Fisher,Cat#PA5-20343; RRID: AB_11153819 Anti-human BRD4,Cell Signaling Technology,Cat#13440; RRID: AB_2687578 Actin,Cell Signaling Technology

(13) Anti-tumor effect of innovative tumor treatment device OM-100 through enhancing anti-PD-1 immunotherapy in glioblastoma growthScientific reportsAugust 8, 2024Yan Z, Huang L, Zhang X, Yu X, Huang R.Protein band intensities were visualized using an ECL kit. The primary antibodies used: PD-L1 (1:1000, #PA5-20343, Thermo), GAPHD (1:1000, #2118, CST, MA, USA). For the sary antibody, a Goat Anti-Rabbit IgG H&L (HRP;

(14) Statins inhibit paclitaxel-induced PD-L1 expression and increase CD8+ T cytotoxicity for better prognosis in breast cancerInternational journal of surgery (London, England)August 1, 2024Li L, Wang H, Zhang S, Gao S, Lu X, Pan Y, Tang W, Huang R, Qiao K, Ning S.Cell Signaling Technology) and GAPDH (2118, Cell Signaling Technology) were employed. Additionally, PD-L1 Antibody (PA5-20343, Thermo Scientific) was utilized in flow cytometry analysis. CD8+ T cell-mediated cancer cell killing

(15) Anticancer effect of propranolol on diethylnitrosamine-induced hepatocellular carcinoma rat modelFundamental & clinical pharmacologyAugust 1, 2024Tamim YM, Nagy AA, Abdellah AM, Osman AH, Ismail AFM.

(16) Peptide Blockers of PD-1-PD-L1 Interaction Reinvigorate PD-1-Suppressed T Cells and Curb Tumor Growth in MiceCellsJuly 15, 2024Zhong SJ, Liu X, Kaneko T, Feng Y, Hovey O, Yang K, Ezra S, Ha SD, Kim S, McCormick JK, et al.[14], 300 µg of lysate was mixed with 5 µg of human anti-PD-1 (#AF1086, R&D) or mouse anti-PD-L1 (#PA5-20343, Invitrogen-Thermo Fisher Scientific) at 4 °C for 2 h followed by 30 min incubation with 20 µL of Pierce™ Protein A/G Magnetic Beads

(17) Bending the Rules: Amplifying PD-L1 Immunoregulatory Function Through Flexible Polyethylene Glycol Synthetic LinkersTissue engineering. Part AApril 1, 2024Racca NM, Dontu A, Riley K, Yolcu ES, Shirwan H, Coronel MM.

(18) Inhibition of UV-Induced Stress Signaling and Inflammatory Responses in SKH-1 Mouse Skin by Topical Small-Molecule PD-L1 BlockadeJID InnovMarch 1, 2024Dickinson SE, Vaishampayan P, Jandova J, Ai YE, Kirschnerova V, Zhang T, Calvert V, Petricoin E 3rd, Chow HS, Hu C, et al.(number 13684), and mouse epidermal lysate blots were probed with Invitrogen PD-L1 antibody (number PA5 – 20343). β-Actin (Cell Signaling Technology, number 4970) was used as a loading control. An anti-rabbit secondary

(19) Gut microbiome-derived butyrate inhibits the immunosuppressive factors PD-L1 and IL-10 in tumor-associated macrophages in gastric cancerGut microbesJanuary 1, 2024Lee SY, Jhun J, Woo JS, Lee KH, Hwang SH, Moon J, Park G, Choi SS, Kim SJ, Jung YJ, et al.Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). The sections were incubated with anti-PD-L1 (PA5–20343; Invitrogen, Carlsbad, CA, USA), anti-IL-10 (BS-20373 R; Bioss Antibodies Inc., Woburn, MA, USA), and

(20) p66Shc deficiency in CLL cells enhances PD-L1 expression and suppresses immune synapse formationFrontiers in cell and developmental biologyJanuary 1, 2024Lopresti L, Capitani N, Tatangelo V, Tangredi C, Boncompagni G, Frezzato F, Visentin A, Marotta G, Ciofini S, Gozzetti A, et al.4°C. Immunoblots were carried out using anti-Shc (Merck Millipore, #06–203), anti-PD-L1 (Invitrogen, #PA5-20343) and anti-actin (Millipore, #MAB1501) primary antibodies. Secondary peroxidase-labeled anti-mouse (#115–035–146)

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PD-L1 Antibody

PD-L1 Antibody from Thermo Fisher Scientific

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PD-L1 Antibody from Thermo Fisher Scientific

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