Fig 1: Effect of octreotide on MUC16, GRHL2, and MACC1 gene expression in GH3 cells. (A) Cells were treated with 0.1 µM and 1 µM octreotide for 4, 8, and 24 h. Gene expression was analyzed by real-time PCR and expressed relative to value from untreated control cells, which was defined as 1. Data were normalized to GUSB and values were calculated using a comparative (2-?Ct) method. Results are mean ± SD from two independent experiments. (B) Western blot analysis of MACC1 protein expression in octreotide treated GH3 cells. ß-actin was used as a loading control. The experiments were performed twice with similar results.