Western blot was performed using Anti-MyD88 Polyclonal Antibody (Product # PA5-19918) and ~34 kDa band corresponding to MyD88 was observed in THP-1, MOLT-4, Jurkat, SK-BR-3, HeLa, Mouse spleen and Mouse lung and not seen in HEK-293 and HT-1080 which are reported to be low expressing cells. Also a lower reported isoform of ~31 kDa and an un-characterized band (*) at ~58 kDa were also observed in the cell lines and tissues. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), THP-1 transfected with 10 µg/mL of Polyinosinic:Polycytidylic acid (Lane 2), MOLT-4 (Lane 3), Jurkat (Lane 4), SK-BR-3 (Lane 5), HeLa (Lane 6), HEK-293 (Lane 7), HT-1080 (Lane 8), Mouse spleen (Lane 9) and Mouse lung (Lane 10) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).