Fig 1: Tspan8 is selectively recruited to 344SQ EVs by ITSN2.(A) Representative Western blots and (B) quantification of 393P and 344SQ WCL expression of ITSN2, CD49d, Tspan8, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C) Representative Western blot and (D) quantification of Western blots of Tspan8, ITSN2, TSG101, HSP70, and GAPDH expression in EVs and WCLs of 393P and 393P cells overexpressing ITSN2 (393PItsn2+). Data are means ± SEM from three independent experiments. **P < 0.01.