Immunofluorescent analysis of MCM7 in paraformaldehyde-fixed HeLa cells using a MCM7 polyclonal antibody (Product # PA5-22104) at a 1:200 dilution.

Western blot was performed using Anti-MCM7 Polyclonal Antibody (Product # PA5-22104) and a 80 kDa band corresponding to MCM7 was observed in all the tested cell models along with an uncharacterized band at ~35 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of PC-3 (Lane 1), LNCaP (Lane 2), DU 145 (Lane 3), HeLa (Lane 4), MCF7 (Lane 5), MKN45 (Lane 6), SW480 (Lane 7), and Jurkat (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1,000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).