Western blot was performed using Anti-GNAS Polyclonal Antibody (Product # PA5-22261) and a 50 kDa band corresponding to GNAS was observed in Jurkat, ACHN, MCF7, OVCAR3, Hep G2, Mouse Brain and Mouse Adipose, but not in A549, MDA-MB-231 and Rat Brain. An uncharacterized band was observed at ~52 kDa. Whole cell lysates (30 µg lysate) of Jurkat (Lane 1), ACHN (Lane 2), A549 (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5), OVCAR-3 (Lane 6), Hep G2 (Lane 7), tissue Lysates (30 µg lysate) of Mouse Brain (Lane 8), Mouse Adipose (Lane 9) and Rat Brain (Lane 10) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).