Western blot was performed using Anti-VAV1 Polyclonal Antibody (Product # PA5-21495) and a 100kDa band corresponding to VAV1 was observed in Raji, MOLT-4, Jurkat, THP-1, Mouse Thymus, and not in Mouse Kidney which is reported negative for VAV1 expression. Whole cell lysates (30 µg lysate) of Raji (Lane 1), MOLT-4 (Lane 2), Jurkat (Lane 3), THP-1 (Lane 4), tissue extracts (30 µg lysate) of Mouse Thymus (Lane 5) and Mouse Kidney (Lane 6) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076)..