Immunocytochemistry-Immunofluorescence analysis of HSD17B4 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: HSD17B4 Polyclonal Antibody (Product # PA5-22367) diluted at 1:500. Blue: Hoechst 33342 staining.

Western blot was performed using Anti-HSD17B4 Polyclonal Antibody (Product # PA5-22367) and an 80 kDa band corresponding to HSD17B4 was observed in all tissue lysates tested except Mouse Brain. Lower expression of SAMHD1 was observed in Mouse Brain as compared to Mouse Testis and Mouse Heart [10.1016/s0960-0760(99)00066-7]. Tissue lysates (30g lysate) of Mouse Testis (Lane 1), Mouse Heart (Lane 2) and Mouse Brain (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Two uncharacterized bands (*) were observed in certain models.