Fig 2: Fertilisation increases PTEN-specific reversible thiol oxidation. A. Protein A mode RedoxiFluor revealed a significant (unpaired t-test, P = 0.0002, n = 6) fertilisation-induced increase in PTEN-specific reversible thiol oxidation in 1-cell zygotes compared to unfertilised eggs in X. laevis. B. Macroscale RedoxiFluor found no significant (unpaired t-test, P = 0.2592, n = 3) difference in PTEN interactome redox state in 1-cell zygotes compared to unfertilised eggs in X. laevis. C. A representative SDS-PAGE gel image showing the PTEN (highlighted) and the redox state (reduced = green channel; oxidised = red channel) of its interactome (arrows 1–6 correspond to coeluting proteins with molecular weights of ~100, 75, 60, 37, 25 and 10 kDa, respectively) in unfertilised eggs (E) and zygotes (Z). D. Quantifying the redox state of the PTEN-specific band manually excised and eluted from (C) in unfertilised eggs and 1-cell zygotes in X. laevis confirmed the significant (unpaired t-test, P = 0.0020, n = 3) fertilisation-induced increase in PTEN-specific reversible thiol oxidation. Data are presented as the mean (M) and standard deviation (SD). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)