Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-12 (Lane 1), Neuro 2a (Lane 2), PC-3 (Lane 3) and tissue extracts of Mouse Lung (Lane 4) and Mouse brain (Lane 5). The blot was probed with Anti- NLK Polyclonal Antibody (Product # PA5-21877, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 56 kDa band corresponding to NLK was observed across all the cell lines and tissues tested except PC-3 cell line.