Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), MCF7 (Lane 2), MOLT-4 (Lane 3), Hep G2 (Lane 4), Jurkat (Lane 5), LNCaP (Lane 6), A549 (Lane 7), Mouse Skeletal Muscle (Lane 8), Rat Skeletal Muscle (Lane 9), Mouse Brain (Lane 10) and Rat Brain (Lane 11). The blot was probed with Anti-beta-1 Adaptin Polyclonal Antibody (Product # PA5-21381, 1:500 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110kDa band corresponding to beta-1 Adaptin was observed across all the cell lines and tissue lysates tested except Mouse Skeletal Muscle and Rat Skeletal Muscle which are reported negative for beta-1 Adaptin expression. An uncharacterized band was also observed at ~80kDa in all the cell lines.