Fig 1: Effects of rosiglitazone intervention on eWAT inflammation.(A) Representative photomicrographs of HPS-stained eWAT cross-sections (magnification x200). (B) High-fat feeding strongly increased CLS formation in eWAT between 9 weeks (REF) and 16 weeks (HFD), while rosiglitazone fully blocked further CLS formation. (C) MCP-1 gene expression was increased in HFD mice, but not in HFD + Rosi. (D) Gene expression of macrophage markers. Rosiglitazone reduced HFD-induced expression of M1 markers (CD11c and CCR2) and increased gene expression of M2 marker Arginase-1 (Arg-1). HFD-induced expression of general macrophage markers, CD68 and F4/80, were not affected by rosiglitazone. (E) Morphometric analysis of average adipocyte size revealed that rosiglitazone attenuated HFD-induced increase in adipocyte size in eWAT. Data are mean ± SEM (n = 7–10/group), *p < 0.05. Mean expression of RT-PCR data was set 1 for chow-fed mice.