RXRalpha (D6H10) Rabbit mAb 3085S from Cell Signaling Technology

Product Specs
ItemRXRalpha (D6H10) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3085S
Quantity100 µl
ApplicationsW, IP, ChIP
ReactivityH, M, R
TargetRXR-alpha
IsotypeIgG
HostRabbit
NCBI Gene AliasesNR2B1, RXR-alpha, RXRalpha
NCBI Full Gene Nameretinoid X receptor alpha
CloneD6H10
Molecular Weight53
ClonalityMonoclonal
Research AreaNuclear Receptor Signaling

Questions and Answers

Can this antibody be used to study RXRα interactions in adipocytes?

Yes, the RXRα (D6H10) Rabbit mAb has been shown to interact with the PPARγ/RXRα protein complex in adipocytes, as demonstrated in a study where co-immunoprecipitation and ChIP-seq assays were utilized to analyze these interactions ().

Is this antibody effective for Western blot applications?

The RXRα antibody has been effectively used in Western blot analyses, as cited in multiple studies, including those examining nitro-alkylated proteins and their interactions in various cellular contexts (, ).

What species can this antibody be used with?

The RXRα antibody is reactive with mouse, human, and rat samples, making it suitable for studies involving these species ().

Are there any known applications of this antibody in cancer research?

Yes, the RXRα antibody has been utilized in research related to acute myeloid leukemia, where it was part of studies investigating the effects of retinoic acid signaling and its interactions with other pathways in cancer cells ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Metabolic stress sensing by epithelial RXRα links westernization of diet with Crohn’s diseaseCell MetabolismMarch 3, 2026Moritz Meyer, Felix Grabherr, Christina Plattner, Michel V. Hadjihannas, Zhigang Rao, Valentin Marteau, Víctor Alonso López-Agudelo, Julian Schwärzler, Lisa Mayr, Almina Jukic, et al.were analyzed with Zen 2012 and ImageJ software. Following antibodies were used: anti-RXRα (1:50; CST, 3085), anti-Lysozyme (1:200; Abcam, ab108508), anti-GPX4 (1:400, Abcam, ab125066). Immunohistochemistry

(2) Functional variants at 1p36.23 confer risk of schizophrenia through modulating RERENature CommunicationsJanuary 24, 2026Yixing Liu, Junyang Wang, Hong Yang, Yifan Li, Changgai Mu, Xinglun Dang, Xinran Chen, Danyang Li, Mingna Li, Jiewei Liu, et al.fragments were then incubated overnight at 4 °C with the indicated antibodies, including IgG, RXRA (CST, 3085S), and RARB (Abcam, ab53161). For each IP reaction, 30 µL of ChIP-grade protein G magnetic beads were

(3) PPARG-centric transcriptional re-wiring during differentiation of human trophoblast stem cells into extravillous trophoblastsNucleic Acids ResJuly 24, 2025Qingqing Guo, Joonhyuk Choi, Muyoung Lee, Jonghwan Kimwere as follows: PPARG (Cell Signaling Technology, 2443S; 1:1000), RXRA (Cell Signaling Technology, 3085S; 1:1000), EP300 (Abcam, ab10485; 1:1000), MED1 (Novus Biologicals, NB100-2574; 1:1000), MED15 (Proteintech,

(4) Cell-state-dependent regulation of PPARγ signaling by the transcription factor ZBTB9 in adipocytesJournal of Biological ChemistryDecember 1, 2024Xuan Xu, Alyssa Charrier, Sunny Congrove, Jeremiah Ockunzzi, David A. Buchneranti-ZBTB9 antibody was from Aviva Systems Biology (#ARP31669_P050). The anti-PPARγ (#2443), anti-RXRα (#3085), anti-phopho-RB (#8180), anti-RB (#9313), anti-Vinculin (#13901) antibodies were from Cell Signaling Technologies

(5) A pan-family screen of nuclear receptors in immunocytes reveals ligand-dependent inflammasome controlImmunityDecember 1, 2024Yutao Wang, Yanbo Zhang, Kyungsub Kim, Jichang Han, Daniel Okin, Zhaozhao Jiang, Liang Yang, Arun Subramaniam, Terry K. Means, Frank O. Nestlé, et al.supernatant was incubated overnight at 4°C on a rotator with 3 μg of anti-RXRα (D6H10) Rabbit mAb (#3085, Cell Signaling) pre-coupled to Protein A Dynabeads (Invitrogen). The beads were washed twice sequentially

(6) A noncoding variant confers pancreatic differentiation defect and contributes to diabetes susceptibility by recruiting RXRANature CommunicationsNovember 12, 2024Yinglei Li, Ran Zheng, Lai Jiang, Chenchao Yan, Ran Liu, Luyi Chen, Wenwen Jin, Yuanyuan Luo, Xiafei Zhang, Jun Tang, et al.Cell Signaling Technology, Cat#54551), anti-FOXA2 (1:200, R&D, Cat#AF2400), anti-RXRA (1:300, Cell Signaling Technology, Cat#3085), anti-C-peptide (1:400, DSHB, Cat#GN-ID4), anti-Glucagon (1:500, Servicebio, Cat#GB11097), anti-Glucagon

(7) Molecular regulation of PPARγ/RXRα signaling by the novel cofactor ZFP407PLOS ONEMay 23, 2024Alyssa Charrier, Jeremiah Ockunzzi, Leighanne Main, Siddharth V. Ghanta, David A. Buchnerand western blotting was performed as previously described [18]. Anti -PPARγ (cat.#: 2430 and 2443), anti-RXRα (cat.#: 3085), and anti-IgG (cat.#: 2729) antibodies were obtained from Cell Signaling (USA). Anti-GAPDH (cat.#:

(8) CALR but Not JAK2 Mutations Are Associated with an Overexpression of Retinoid X Receptor Alpha in Essential ThrombocythemiaCancers (Basel)April 16, 2024Ana Guijarro-Hernández, Cristina Hurtado, María José Larráyoz, María José Calasanz, José Luis Vizmanosfor the detection of each protein, diluted in the same solution prepared for blocking: anti-RXRα (#3085, Cell Signaling Technology®, Danvers, MA, USA), diluted 1:1000 in 5% milk; anti-PPARγ (#2435, Cell Signaling Technology

(9) Raising NAD + Level Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein DeacetylationCirculationDecember 19, 2023Matthew A. Walker, Hongye Chen, Aprajita Yadav, Julia Ritterhoff, Outi Villet, Tim McMillen, Yuliang Wang, Hayley Purcell, Danijel Djukovic, Daniel Raftery, et al.(Cell Signaling #2729) was used as a replacement for the RXRα rabbit antibody (Cell Signaling D6H10 #3085). The ChIP procedure was performed as in the supplier’s protocol (EZ-ChIP EMD Millipore catalog # 17–371)).

(10) Netrin-1 feedforward mechanism promotes pancreatic cancer liver metastasis via hepatic stellate cell activation, retinoid, and ELF3 signalingCell reportsNovember 28, 2023Dudgeon C, Casabianca A, Harris C, Ogier C, Bellina M, Fiore S, Bernet A, Ducarouge B, Goldschneider D, Su X, et al.and western blotting, rabbit polyclonal antibodies against RXRα and ELF3 were purchased from Cell Signaling (3085S) and Invitrogen (PA5-89261), respectively, and compared with normal mouse IgG purchased from Cell Signaling

(11) Raising NAD+ level stimulates short-chain dehydrogenase/reductase proteins to alleviate heart failure independent of mitochondrial protein deacetylationCirculationNovember 15, 2023Matthew A. Walker, Hongye Chen, Aprajita Yadav, Julia Ritterhoff, Outi Villet, Tim McMillen, Yuliang Wang, Hayley Purcell, Danijel Djukovic, Daniel Raftery, et al.(Cell Signaling #2729) was used as a replacement for the RXRα rabbit antibody (Cell Signaling D6H10 #3085). The ChIP procedure was performed as in the supplier’s protocol (EZ-ChIP EMD Millipore catalog # 17–371)).

(12) Characterizing 3T3-L1 MBX Adipocyte Cell Differentiation Maintained with Fatty Acids as an In Vitro Model to Study the Effects of ObesityLife (Basel)August 9, 2023Noshin Mubtasim, Lauren Gollahon3305), PPAR-γ Rabbit mAb (Product# 2435) SirT1 Rabbit mAb (Product # 2496), RXRα Rabbit mAb (Product# 3085) primary antibodies from Cell Signaling Technology were used at 1:1000 dilution for overnight incubation.

(13) Stem cells tightly regulate dead cell clearance to maintain tissue fitnessbioRxiv : the preprint server for biologyMay 22, 2023Stewart KS, Gonzales KA, Yuan S, Tierney MT, Bonny AR, Yang Y, Infarinato NR, Cowley CJ, Levorse JM, Pasolli HA, et al.1:250), rat biotinylated anti-CD45 (Biolegend, 5530, 1:200), rabbit anti-RXRα (D6H10) (Cell Signalling, 3085, 1:250), rabbit anti-RARγ (D3A4) (Cell Signaling, 8965, 1:250), rabbit anti-MFGE8 (Invitrogen, PA5–109955,

(14) STRA6 is essential for induction of vascular smooth muscle lineages in human embryonic cardiac outflow tract developmentCardiovascular researchMay 22, 2023Zhou C, Häneke T, Rohner E, Sohlmér J, Kameneva P, Artemov A, Adameyko I, Sahara M.(Cell Signaling Technology, 8884S); anti-RARα (Cell Signaling Technology, 62294S); anti-RXRα (Cell Signaling Technology, 3085S); anti-STRA6 (Novus, NBP1-83719); and anti-TBX1 (Abcam, ab18530) (see Supplementary material online,

(15) 1α, 25-Dihydroxyvitamin D3 protects gastric mucosa epithelial cells against Helicobacter pylori-infected apoptosis through a vitamin D receptor-dependent c-Raf/MEK/ERK pathwayPharmaceutical BiologyDecember 31, 2022Shuai Zhao, Daihong Wan, Yaoyao Zhong, Xiwei Xu(#4694), anti-phospho-MEK1/2 (#9154), anti-ERK1/2 (#4695), anti-phospho-ERK1/2 (#4370), anti-RXRα (#3085), anti-VDR (#12550), anti-GAPDH (#97166) and secondary anti-mouse or anti-rabbit antibodies were purchased

(16) Human Cytomegalovirus Induces Vitamin-D Resistance In Vitro by Dysregulating the Transcriptional Repressor SnailVirusesSeptember 10, 2022Stecher C, Maurer KP, Kastner MT, Steininger C.SuperSignal Pico or Femto substrates from Thermofisher Scientific. The monoclonal antibodies RXRα #3085, Snail #3879, Slug #9585, VDR #12550, polyclonal antibody against DNA-PKcs #4602 and secondary goat

(17) Gene repression through epigenetic modulation by PPARA enhances hepatocellular proliferationiScienceMay 20, 2022Aibara D, Takahashi S, Yagai T, Kim D, Brocker CN, Levi M, Matsusue K, Gonzalez FJ.1:500), anti-CDH1 (Cell Signaling Technology; #3195, 1:1000), anti-RXRA (Cell Signaling Technology; #3085, 1:1000), anti-SETDB1 (Proteintech; 11231-1-AP, 1:1000) or anti-HDAC1 (Abcam; ab7028, 1:1000) rabbit

(18) The ShGlomAssay Combines High-Throughput Drug Screening With Downstream Analyses and Reveals the Protective Role of Vitamin D3 and Calcipotriol on PodocytesFrontiers in Cell and Developmental BiologyMay 16, 2022Marie-Christin Ristov et al.Cell Signaling Technology, Frankfurt am Main, Germany #12550) and rabbit anti-RXRa (1:500, Cell Signaling Technology #3085). Samples were run in Stain-free TGX gels and quantified with Criterion Stain Free Imager (SFI, Bio-Rad Laboratories

(19) B-cell lymphoma 6 alleviates nonalcoholic fatty liver disease in mice through suppression of fatty acid transporter CD36Cell death & diseaseApril 18, 2022Zhang H, Li Y, Zhang C, Huang K, Zhao J, Le S, Jiang L, Liu H, Yang P, Xiao X, et al.(Affinity, DF3845), anti-CD36 (Cell Signaling Technology, 14347), anti-RXR (Cell Signaling Technology, 3085), anti-PXR (Abcam, ab192579), anti-LXRα (Proteintech,14351-1-AP), anti-LXRβ (Cell Signaling Technology,

(20) Mechanisms involved in suppression of osteoclast supportive activity by transforming growth factor-β1 via the ubiquitin-proteasome systemPloS oneJanuary 1, 2022Inoue M, Nagai-Yoshioka Y, Yamasaki R, Kawamoto T, Nishihara T, Ariyoshi W.transcription3 (STAT3) monoclonal (#4904), anti-phospho-STAT3 polyclonal (#9131), anti-RXR-α monoclonal (#3085), anti-ubiquitin monoclonal (#3936), and anti-Histone H3 polyclonal antibodies (#9715) were purchased

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RXRalpha (D6H10) Rabbit mAb

RXRalpha (D6H10) Rabbit mAb from Cell Signaling Technology

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Questions and Answers

Cell Signaling Technology

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RXRalpha (D6H10) Rabbit mAb from Cell Signaling Technology

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