IF LAP2 alpha Monoclonal Antibody [3A3] on HeLa cells

LAP2 alpha antibody [3A3]

Hela cells were boiled in Laemmli buffer and the proteins were separated by 8% SDS-PAGE and blotted onto nitrocellulose membrane. The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C o/n with anti LAP2α, clone 3A3 antibody at the indicated dilution in 0.5% NFDM/PBS-T. After incubation with anti-mouse Fc-HRP coupled secondary antibody for 1h at RT, ECL was performed with the Western Lightning reagents. A 30sec exposure is shown.