Fig 1: Effects of RRP15 knockdown or overexpression on epithelial-mesenchymal transition (EMT) of CC cells. (A) Representative images of IHC staining of RRP15, E-cadherin and N-cadherin in CC tissues and paired adjacent tissues (scale bar = 100 μm). (B) Effects of RRP15 knockdown on the expression of E-cadherin in HCT15 cells examined by Western blot. Representative blots via densitometry. (C) Effects of RRP15 knockdown on the expression of N-cadherin in HCT15 cells via Western blot. Representative blots are shown with densitometry (n = 3). (D) The effect of RRP15 overexpression on expression of E-cadherin in HCT116 cells was examined by Western blot. Representative blots are shown with densitometry. (E) Effects of RRP15 overexpression on the N-cadherin expression in HCT116 cells by Western blot. Representative blots showed under densitometry. * p < 0.05 and ** p < 0.01 versus the control shRNA group; # p < 0.05 and ## p < 0.01 versus the control vector group, n = 3.
Fig 2: Effects of RRP15 knockdown or overexpression on CC cell invasion. (A) Effects of RRP15 knockdown on HCT15 cell invasion (scale bar = 100 μm). (B) Quantitation of the number of invasion cells. (C) Effects of RRP15 overexpression on HCT116 cell invasion (scale bar = 100 μm). (D) Quantitation of the number of invasion cells. ** p < 0.01 versus the control shRNA group; ## p < 0.01 versus the control vector group, n = 3.
Fig 3: The implication of RRP15 knockdown or overexpression on the cellular proliferation and colony formation of CC cells. (A) Western blot analysis of RRP15 expression level in HCT15 cells after RRP15 shRNA plasmid transfection. (B) Western blot analysis of RRP15 expression level in HCT116 cells after RRP15 pcDNA3.1(+) plasmid transfection. Effects of RRP15 knockdown on the proliferation of (C) HCT15 cells and (D) HCT116 cells via CCK8. Effects of RRP15 knockdown on the colony-forming ability of (E) HCT15 cells and (F) HCT116 cells. (G,H) Quantitation of the colony numbers. ** p < 0.01 and *** p < 0.001 versus the control shRNA group; ## p < 0.01 versus the control vector group, n = 3.
Fig 4: RRP15 expression in human colon cancer (CC) samples and correlation with patient survival. (A) Representative images of RRP15 via immunohistochemical (IHC) (scale bar = 100 μm). (B) RRP15 protein expression in eight paired CC tissues (marked C) and matched normal tissue samples (marked N) was determined by Western blot. (C) Quantitation of Western blot results. The relationship between RRP15 expression and (D) the overall survival (OS) and (E) disease-free survival (DFS) of CC patients. ** p < 0.01 versus the N group, n = 8.
Fig 5: Effects of RRP15 knockdown or overexpression on tumor growth of CC cells. (A) Representative images of tumor-bearing nude mice subcutaneously injected with HCT116 cells transfected with RRP15 pcDNA3.1(+) plasmid. (B) Images for tumor xenografts from nude mice. (C) Tumor weight and (D) volume. (E) Representative images of tumor-bearing nude mice that were subcutaneously injected with HCT15 cells transfected with RRP15 shRNA plasmid. (F) Images for tumor xenografts from nude mice were shown. (G) Tumor weight and (H) volume. # p < 0.05 and ## p < 0.01 versus the control or control vector group; * p < 0.05 versus the control or control shRNA group, n = 5.
Supplier Page from Abcam for Anti-RRP15 antibody