Western blot analysis of RPL27A on different lysates with Rabbit anti-RPL27A antibody (ER64831) at 1/5,000 dilution. Lane 1: A549 cell lysate (15 µg/Lane) Lane 2: 293T cell lysate (15 µg/Lane) Lane 3: HeLa cell lysate (15 µg/Lane) Lane 4: 3T3-L1 cell lysate (15 µg/Lane) Lane 5: Rat pancreas tissue lysate (15 µg/Lane) Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64831) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.