Fig 1: Expression of EphA5, IGFBP5 and H2BK in solid human glioblastoma (GBM) samples(A) Solid human glioma samples of different tumor entities were analysed by qRT-PCR regarding the expression of EphA5, IGFBP5 and H2BK (A = astrocytoma, GBM = glioblastoma). Lines represent the mean gene expression for each gene and tumor entity (?CT 3.3 = 10-fold expression difference; black-marked rhombs correspond to cultured GBM cells in Figure 3A). (B and C) Solid human glioblastoma sections were stained by double-immunohistochemistry regarding the expression of IGFBP5, EphA5 and H2BK to identify dormant cells. Sections were also stained by double-immunohistochemistry with combinations of (B) GFAP, vWF or (C) Ki67 and with IGFBP5, EphA5, H2BK themselves (white bars indicate 20 µm).