Fig 1: Impact of BRCC3 inactivation on cytokine release. a Top up-regulated cytokines in Kasumi-1 BRCC3 KO supernatant compared to BRCC3 WT assessed via Cytokine Array. b Western blot depicting G-CSF levels in Kasumi-1 BRCC3 WT and BRCC3 KO cells. Growth curves of c BRCC3 WT Kasumi-1 and d SKNO-1, e BRCC3 KO Kasumi-1, and f BRCC3 WT ME-1 cells treated with 10 ng/mL G-CSF or a vehicle control for 8 days. g Kasumi-1 BRCC3 WT and BRCC3 KO cells treated with a neutralizing anti-G-CSF antibody for 13 days. h Colony forming unit (CFU) assay of AML1-ETO and AML1-ETO + Brcc3 KO cells treated with G-CSF or a vehicle control. nsNot significant, *P < 0.05, **P < 0.01, ***P < 0.001. All p-values are calculated using unpaired Student t test. Error bars are standard error of the mean. n = 2 for Fig. 5h, n = 3 for all other experiments for data shown, which are representative of two or three independent experiments respectively