Fig 2: Histological analysis of marker genes.Protein expression was determined by immunohistochemistry using sections from formalin-fixed, paraffin-embedded tissue. Proteins explored were CDKN3A, SYCP2, PRC1, CDC20, CCNB2, PCNA, CDKN2A, MKI67, and CDC2. Representative experiments in adeno cell carcinomas (panel A) and squamous cell carcinomas (panel B) are shown. The specific signals are shown as brown staining (counterstained with hematoxylin; original magnification, ×400; bars, 10 µm).

Fig 3: Validation of gene expression of 9 genetic markers by qRT-PCR.The intensity of gene expression, expressed in Log2 values, is shown in box plots. Expression of the 6 genes validated in this study (CCNB2, PRC1, SYCP2, CDKN3, CDC20, and NUSAP1) and the 3 well-known genes (CDKN2A, MKI67, and PCNA) associated with CC are compared among the 4 groups, including healthy cervical epitheliums (Normal, n = 25), low-grade CIN (CIN1, n = 29), high-grade CIN (CIN2/3, n = 21), and invasive CC (cancer, n = 44). The upper and lower boundaries of the boxes represent the 75th and 25th percentiles, respectively. The black line within the box represents the median value, and the whiskers represent the minimum and maximum values that lie within 1.5× the interquartile range from the end of box. Values outside this range are represented by black circles. The fold change (FC) was calculated by dividing the median of each pathological group by the median of the control group.

Fig 4: Augmin contributes to k-fiber and interpolar microtubule formationImages of control and HAUS6-depleted cells acquired by CH-STED nanoscopy.(A) Immunofluorescence of IM fibroblasts using paraformaldehyde and glutaraldehyde fixation. DAPI, a-tubulin, and ACA are shown in inverted grayscale. Astral MT tracks are represented in magenta (tracing). Scale bar: 5 µm.(B) Quantification of astral MT length (n = 665 control astral MTs/10 cells; n = 750 siHAUS6 astral MTs/11 cells). The boxplot determines the interquartile range; the line inside the box represents the median; data pooled from three independent experiments and analyzed using an unpaired t test; ****p = 0.0001.(C) Immunofluorescence of IM fibroblasts using cold methanol fixation. ß-Tubulin (magenta) and PRC1 (green). Scale bar: 5 µm.(D) 3D representations of mitotic spindles in control and HAUS6-depleted cells, illustrating KT surfaces 1 and 2 (magenta), as well as the plates that define the measurement volumes, corresponding to interpolar MTs (ipMTs, green) and k-fibers (kMTs) plus ipMTs (cyan).(E) Quantification of ipMTs and kMTs in control and HAUS6-depleted cells. Proportion relative to control levels is represented for HAUS6-depleted cells (n = 14 control cells; n = 12 siHAUS6 cells). Data pooled from two independent experiments and analyzed using an unpaired t test; ****p = 0.0001.