FXR (D-3) Antibody sc-25309 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemFXR (D-3) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-25309
Antibody TypePrimary Antibodies
NCBI Gene AliasesBAR, Rxrip14, HRR-1, HRR1, RIP14, Fxr, PFIC5, FXR
Quantity200 µg/ml
CloneD-3
ApplicationsWB, IP, IF, ELISA
Reactivitymouse, rat, human
ClonalityMonoclonal
Conjugate/TagUnconjugated
Hostmouse
FormatPurified
NCBI Full Gene Namenuclear receptor subfamily 1, group H, member 4, nuclear receptor subfamily 1 group H member 4
TargetFXR
IsotypeIgG2a

Questions and Answers

What applications has the FXR (D-3) Antibody been used for in scientific research?

The FXR (D-3) Antibody has been shown to work in various applications, including Western Blotting, ELISA, Immunofluorescence, and Immunoprecipitation. For instance, it has been utilized in studies examining the expression of FXR in human hepatocytes and its role in signaling pathways related to bile acid metabolism and liver function.

Can this antibody be used to study FXR's role in cancer research?

Yes, the FXR (D-3) Antibody has been referenced in studies investigating the oncogenic function of NR1H4 (FXR) in clear cell renal cell carcinoma, where it was used to assess the expression levels of FXR and its correlation with cancer cell proliferation and migration.

Is the FXR (D-3) Antibody effective in animal models?

The FXR (D-3) Antibody has been effectively used in animal models, such as in studies involving rats to explore the protective effects of cilostazol against estrogen-induced intrahepatic cholestasis, where FXR signaling was a key focus.

What species does the FXR (D-3) Antibody react with?

The FXR (D-3) Antibody is reactive with multiple species, including humans, mice, and rats, making it suitable for a wide range of biological studies across different model organisms.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Polysaccharide from Walnut Green Husk alleviates ochratoxin a-induced anorexia in chicks via m6A-mediated FXR regulation of orexigenic genesPoultry ScienceMarch 1, 2026Kangyan Zhao, Jiyang Hu, Lishan Jiang, Ruqian Zhao, Deyun Wang, Yang YangChromatin was sheared to ∼300 bp fragments and immunoprecipitated overnight at 4°C with anti-FXR antibody (sc-25309, Santa Cruz) or control IgGs. Protein G beads (sc-2003) captured immune complexes, followed by crosslink

(2) Antibiotic cocktail-induced changes in gut microbiota drive alteration of bile acid metabolism to restrain Th17 differentiation through the FXR–NLRP3 axisGut MicrobesDecember 31, 2025Yue Shao-Yu, Di Niu, Jia Chen, Wei-Yi Li, Xu Wang, Qing-Wei Meng, Wei-Jie Song, Yi-Ge Yang, Hui Wang, Rong-Rong Li, et al.with mouse anti-IL-17A antibodies (Proteintech, 66148−1-lg), rabbit anti-FXR antibodies (Santa Cruz, sc−25309), rabbit anti-caspase-1β antibodies (Affinity, AF5418), rabbit anti-ASC antibodies (Affinity, DF6304),

(3) Smilax china L. Extract Alleviates Metabolic-Associated Fatty Liver Disease by Regulating Gut Microbiota and Bile Acid MetabolismMetabolitesDecember 26, 2025Shiyuan Cheng, Huijun Li, Zhiying Sun, Yue Xiong, Jing Li, Jiaying Tian, Yue Shen, Li Shen, Jingyu Yang, Yuying Yang, et al.acetonitrile, and methanol came from Sigma-Aldrich (Darmstadt, Germany). Primary antibodies of FXR (Cat. SC-25309, 1:1000), CYP7A1 (Cat. SC-518007, 1:1000) were obtained from Santa Cruz Biotechnology Co., Ltd. (Dallas,

(4) Unlocking the potential of atractylenolide II: Mitigating non-alcoholic fatty liver disease through farnesoid X receptor-endoplasmic reticulum stress interplayJournal of Pharmaceutical AnalysisDecember 1, 2025Ming Gu, Zhiwei Chen, Yujun Chen, Yiping Li, Hongqing Wang, Ya-ru Feng, Peiyong Zheng, Cheng Huangdetected using antibodies against ACTB (Cat# 3700, Cell Signaling Technology, Danvers, MA, USA), FXR (Cat# sc-25309, Santa Cruz Biotechnology, Santa Cruz, CA, USA), SERCA2 (Cat# A1097, ABclonal), small heterodimer partner

(5) β-Catenin–Cohesin Ring–CEGRs/ALCDs Axis Activation Contributes to the Development of Hepatoblastoma and Fibrolamellar HCCMolecular Cancer ResearchNovember 3, 2025Yasmeen Fleifil, Ruhi Gulati, Katherine Jennings, Ina Kycia, Alex Bondoc, Gregory Tiao, James Geller, Matthew Weirauch, Khashayar Vakili, Lubov Timchenko, et al.Anti-neurotensin (Millipore Sigma, SAB42000703), β-actin (Santa Cruz, sc-47778), FXR, (Santa Cruz, sc-25309) and NGF (Abcam, ab216419). We generated a new J-PKAc fusion-specific antibody (referred to as Fus-Ab

(6) Gut microbiota-bile acid metabolic disorder involved in the cognitive impairments in epilepsy through HO-1 dependent ferroptosisJournal of Pharmaceutical AnalysisNovember 1, 2025Xinyu Li, Jia Ji, Jing Li, Saisai Li, Qiang Luo, Maosheng Gu, Xin Yin, Meng Zhang, Hongbin Fan, Ruiqin Yao(Proteintech, 22401-1-AP, Chicago, IL, USA), anti-4HNE (Abcam, ab46545, Cambridge, UK), anti-FXR (SANTA CRUZ, sc-25309, Santa Cruz, CA, USA), anti-Hmox1 (Proteintech, 10701-1-AP, Chicago, IL, USA), anti-Nrf2 (Proteintech,

(7) Gut microbiota composition combined with reduced intestinal fatty acid uptake prevents hepatic steatosis in obesity-resistant mice fed a high-fat dietScientific reportsOctober 23, 2025Vratarić M, Teofilović A, Vojnović Milutinović D, Veličković N, Đmura G, Djordjevic A.sc-366, Santa Cruz Biotechnology), anti-ChREBP (1:1000, NB400-135, Novus Biologicals), anti-FXR (1:500, sc-25309, Santa Cruz Biotechnology), anti-PPARα (1:500, sc-398394, Santa Cruz Biotechnology), anti-PGC-1α (1:500,

(8) Sodium Butyrate Ameliorated Bile Acid Metabolism in Diabetes Mellitus by PI3K/AKT Signaling Pathway via the Gut–Liver AxisCurrent Issues in Molecular BiologySeptember 9, 2025Tingting Zhao, Xi Zhang, Qian Xiang, Yadi Liu, Xuling Li, Junling Gu, Wenqian Zhang, Zhe Wang, Yiran Li, Xiaoshan Lai, et al.(AF5001) was obtained from the Beyotime Company (Shanghai, China). Antibodies against AKT (sc-5298), FXR (sc-25309), p-AKT (sc-293125), CYP7A1 (sc-518007), GSK3α/β (sc-7291), and p-GSK3α/β (sc-81496) were purchased

(9) Targeting pregnane X receptor with a potent agonist-based PROTAC to delay colon cancer relapseOncogenesisAugust 30, 2025Lucile Bansard, Guillaume Laconde, Vanessa Delfosse, Tiphaine Huet, Margaux Ayeul, Emilie Rigal, Quentin Donati, Sabine Gerbal-Chaloin, Martine Daujat-Chavanieu, Luc Brunel, et al.antibodies were used with catalog number and final dilutions shown: GADPH (sc-32233, 1/5000), FXR (sc-25309, 1/500), VDR (sc-1009, /1000), RXR-alpha (sc-515929, 1/500), CYP3A4 (sc-53850, 1/500), Ubiquitin (sc-166553,

(10) Cholecystectomy-related gut microbiota dysbiosis exacerbates colorectal tumorigenesisNat CommunAugust 16, 2025Bo Tang, Shengpeng Li, Xin Li, Jialin He, An Zhou, Lingyi Wu, Xu Xiao, Sumin Wang, Hongfei Jiang, Jincheng Jian, et al.Biomedicals (Shanghai) Co., Ltd. Antibodies used were as follows: FXR monoclonal antibody (Santa cruz, sc-25309), β-catenin monoclonal antibody (Proteintech, 66379-1-Ig), c-Myc monoclonal antibody (Abcam, ab32072),

(11) DHLCA Alleviates Diabetic Kidney Disease via TGR5/FXR Activation and Gut Microbiota RemodelingDrug Design, Development and TherapyJuly 29, 2025Hua Zhou, Xiaodie Mu, Huiyue Hu, Shuya Zhao, Nan Hu, Min Yang, Jingting Jiangto WB analysis. Prior to incubation with primary antibodies against TGR5 (ab72608, Abcam, USA), FXR (sc-25309, Santa Cruz, USA), and β-actin (MA1-140, Invitrogen, USA) overnight at 4°C, the nitrocellulose membranes

(12) The role of gut microbiota on skeletal muscle strength in LDLR knockdown aging miceSci RepMay 2, 2025Ying Li, Ziyang Fang, Yinjun Luo, Huiling Liu, Dianchen Zhao, Tao Feng, Xin Zhang, Songyi Mo, Xiaoyan Fang, Lingling Huang, et al.primary antibodies overnight at 4 °C, respectively. Including ASBT (ab203205, 1:1000; Abcam), FXR (sc-25309, 1:1000; Santa Cruz), FGF15 (sc-398338, 1:1000, Santa Cruz), HSP90 (sc-13119, 1:2000; Santa Cruz), FGFR4

(13) Lactococcus petauri LZys1 modulates gut microbiota, diminishes ileal FXR-FGF15 signaling, and regulates hepatic functionMicrobiology SpectrumApril 17, 2025Ouyang Li, Yingshun Zhou, Dayoung Kim, Han Xu, Zhijun Bao, Fan YangTween 20 (TBST) at room temperature for 2 h and then incubated with FXR (Santa Cruz Biotechnology, sc-25309, 1:100), FGFR4 (Santa Cruz Biotechnology, sc-136988, 1:100), and β-actin (CST, 12262, 1:100). After

(14) The farnesoid X receptor activates transcription independently of RXR at non-canonical response elementsNucleic Acids ResearchFebruary 8, 2025Suzanne W van der Veen, Jelmer J Dijkstra, Ellen C L Willemsen, René Houtman, Alexandra Milona, Nikolas Marchet, Maureen Spit, Danielle Hollman, Fried J T Zwartkruis, Michiel Vermeulen, et al.0.15 M NaCl, pH 7.6) with 0.05% Tween-20, 0.2% BSA, 50 μM DTT, 10 μM OCA, FXR antibody (Santa Cruz, #sc-25309) and Donkey anti mouse IgG Alexa Fluor 488 (Invitrogen, #A21202). Incubation was performed for 40 min

(15) FXR activation reduces the formation of macrophage foam cells and atherosclerotic plaque, possibly by down regulating hepatic lipase in macrophagesFEBS open bioFebruary 1, 2025Gu Q, Liu J, Shen LL.Sonics VCX 130 sonicator (Sonics & Materials Inc, Newtown, CT, USA). An antibody directed against FXR (sc‐25309; 5 mg per 1 mg total protein) and antibodies directed against IgG (sc‐66931; 10 mL per 1 mg total protein)

(16) Overexpression of TNFα in TNF∆ARE+/- mice increases hepatic periportal inflammation and alters bile acid signaling in miceHepatology communicationsDecember 1, 2024Shearn CT, Anderson AL, Devereaux MW, Koch SD, Larsen LD, Spencer LA, Orlicky DJ, Colgan SP, Steiner CA, Sokol RJ.Invitrogen/Thermofisher), BSEP (1:1000, catalog number NBP1-89319, Novus Biologicals), FXR (1:1000, catalog number sc-25309, Santa Cruz Biotechnologies), and NTCP (1:1000, catalog number PA5-80001, Invitrogen/Thermofisher).

(17) FXR deficiency induced ferroptosis via modulation of the CBP-dependent p53 acetylation to suppress breast cancer growth and metastasisCell Death & DiseaseNovember 14, 2024Ping Huang, Han Zhao, Hua Dai, Jinying Li, Xiafang Pan, Wentian Pan, Chunhua Xia, Fanglan Liufrom Cell Signaling Technology (14793, MA, USA). Monoclonal antibodies against CBP(sc-365387) and FXR(sc-25309) were purchased from Santa Cruz Biotechnology (CA, USA). Clinical specimens Breast cancer tumors and

(18) Gut microbiota depletion and FXR inhibition exacerbates zonal hepatotoxicity of sunitinibTheranosticsOctober 28, 2024Qi Zhao, Yingmei Lu, Jingyi Duan, Dan Du, Qianlun Pu, Fei Li(LC3B, ab192890, Abcam, UK), zonula occludens-1 (ZO-1, ab96587, Abcam, UK), FXR (NB400-153, Novus), FXR (sc-25309, Santa Cruz Biotechnology, USA), BSEP (PB0379, BOSTER), sequestonsome1 (SQSTM1/P62, ab109012, Abcam,

(19) Protective effects of cilostazol via the HNF1α/FXR signalling pathway and anti-apoptotic mechanisms in a rat model of estrogen-induced intrahepatic cholestasisScientific ReportsOctober 1, 2024Marwa Hassan, Maha B. Salem, Olfat A. Hammam, Mohamed ElZallatwith primary antibodies against HNF1α (Abcam, USA; CAT no: ab128912), FXR (Santa Cruz, USA; CAT no: sc-25309), CYP3A1 (Santa Cruz, USA; CAT no: sc-53246), BSEP (Proteinintech, USA; CAT no: 67512-1-lg), and β-actin

(20) Hammerhead-type FXR agonists induce an enhancer RNA Fincor that ameliorates nonalcoholic steatohepatitis in miceeLifeApril 15, 2024Chen J, Wang R, Xiong F, Sun H, Kemper B, Li W, Kemper J.and immunoprecipitation using control IgG or FXR antibody (Novus Biologicals, NBP2-16550; Santa Cruz, sc-25309), RXRα antibody (Proteintech, catalog no. 21218-1-AP), or BRD4 antibody (Bethyl Laboratories, catalog

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FXR (D-3) Antibody

FXR (D-3) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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FXR (D-3) Antibody from Santa Cruz Biotechnology, Inc.

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