Fig 1: ZDHHC17 mediates palmitoylation of SARM1 in myocardial fibroblasts after MI. (A, B) Venn diagram was used to screen the palmitoyl acylase acting on SARM1 (https://www.proteinatlas.org/). (C) qRT-PCR is used to detect the knockdown efficiency of Zdhhc3, Zdhhc9, Zdhhc16, Zdhhc17, and Zdhhc21. n = 4–6. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. siNC. (D) Western blot was used to detect the protein expression of SARM1 after the deletion of candidate palmitoylase. n = 3. ∗∗P < 0.01 vs. CTL, ##P < 0.01 vs. TGF-β1. (E) Click-iT reaction was used to detect the palmitoylation level of SARM1. n = 3. (F) qRT-PCR is used to detect the expression of Zdhhc17 in fibroblasts after TGF-β1 treatment. n = 5. ∗P < 0.05 vs. CTL. (G) CCK8 to detect the effect of cell viability. n = 12. ∗∗∗P < 0.001 vs. CTL, ###P < 0.001 vs. +siNC. (H) The hydroxyproline assay kit is used to detect the secretion of collagen. n = 4. ∗∗∗P < 0.001 vs. CTL, ###P < 0.001 vs. +siNC. (I) Multiplex immunohistochemistry is used to detect coexpression between ZDHHC17 and SARM1 in myocardial fibroblasts in the infarct margin area of MI tissue. n = 3. Scale bar, 200 μm. Data are presented as mean ± SEM.