Fig 2: Retinal flat mounts and cryostat sections of the eyeball in the AAV2-Pim-1 infected rat were stained with immunofluorescence histochemistry. (A1, B1, C1, D1) AAV2-Pim-1 infected the retina and RGCs. (A2, B3, C3) Blue staining indicates DAPI labeled nuclei. (A3, B4, C4, D3) AAV2-Pim-1 was co-located with ?-synuclein, GFAP or HPC-1. Green fluorescence for AAV2-Pim-1 and red immunostaining for ?-synuclein, GFAP or HPC-1. Scale bar=50 µm, n=4.

Fig 3: FGF21 promoted insulin secretion along with up-regulation of SNARE proteins. Sixteen-week-old db/db mice and their lean controls were intraperitoneally injected with AAV-F21 or AAV-GFP and killed 4 weeks later. (A) The plasma insulin levels were measured immediately following the killing. (B) Static glucose-stimulated insulin secretion (GSIS) in isolated islets was measured at 1 h after 16.7 mmol/L glucose stimulation. (C–E) Quantitative PCR analysis of STX1, SNAP25 and VAMP2 in isolated islets. (F–H) Western blot analysis of STX1, SNAP25 and VAMP2 in isolated islets. # P < 0.05, ## P < 0.01 and ### P < 0.001 vs the lean controls; *P < 0.05 and **P < 0.01 vs db/db mice treated with AAV-GFP. (A) n = 5. (B–H) n = 3-5

Fig 4: STX1 (A), INSM1 (B), CGA (C) and SYP (D) immunoreactivity in a solid papillary carcinoma 20x.