GAL4 [DBD] (RK5C1) Antibody sc-510 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemGAL4 [DBD] (RK5C1) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-510
Antibody TypePrimary Antibodies
ApplicationsWB, IP
Quantity200 µg/ml
CloneDBD
ClonalityMonoclonal
Hostmouse
Conjugate/TagUnconjugated
FormatPurified
TargetGAL4
IsotypeIgG2a

Questions and Answers

Can this GAL4 antibody be used for immunoprecipitation in my experiments?

Yes, the GAL4 [DBD] (RK5C1) antibody has been shown to work effectively in immunoprecipitation applications, as evidenced by its use in various studies, including those that involve co-immunoprecipitation techniques to analyze protein interactions.

What cell lines have been successfully used with this antibody for Western blotting?

The GAL4 antibody has been utilized in Western blotting with several cell lines, including HEK293T and Xenopus laevis A6 kidney cells, as demonstrated in published research. This suggests its reliability for detecting GAL4 protein in these contexts.

Is this antibody suitable for use in immunofluorescence microscopy?

Yes, the GAL4 antibody has been employed in immunofluorescence microscopy, where it was used to stain cells for visualization of GAL4 protein localization, indicating its effectiveness in this application.

What is the host species for this antibody, and how does that affect its use in experiments?

The GAL4 antibody is derived from mouse, which means it is a mouse monoclonal antibody. This is important for experimental design, as it allows for compatibility with various secondary antibodies that are designed to detect mouse IgG, facilitating multiple applications in research.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Identification and validation of an interaction between the E3 Ubiquitin Ligase WWP1 and the transcriptional co-activator WBP2 in the human heartBiology OpenApril 15, 2026Meaghan E. Arnold, Ymani Wright, Natalie K. Grantham, Douglas L. Pittman, Lydia E. Matesicprimary antibodies (mouse anti-yeast GAL 4 DNA-binding domain, clone RK5C1, Santa Cruz Biotechnology #sc-510, RRID:AB_627655, 1:200; mouse anti-human WWP1, Abnova #H00011059-M01, RRID:AB_509107, 1:500; rabbit

(2) A simple and robust reporter-based framework for deep functional characterization of PPARγ mutantsEndocrinologyApril 7, 2026Rosalie Baak, Denise Westland, Eline de Lange, Rene Houtman, Eric KalkhovenTween-20, 0.2% bovine serum albumin, 50-μM DTT, 1-μM rosiglitazone, and Gal4 antibody (Santa Cruz, sc-510; RRID: AB_627655). Incubation was performed for 40 minutes at 20 °C, followed by washing to remove unbound

(3) Comparison of activities of transcription factor NF-κB from two jellyfish modelsComparative Immunology ReportsDecember 1, 2025Leah M. Williams, Wei Wang, Alexandra V. Grigoryeva, Alejandra Navarro-Rosado, Jada A. Peart, Angela Calderon, Catherine L. Gill, Susan Black, Kristina M. Alsante, Aidan T. Lackstrom, et al.(1:1000, Santa Cruz Biotechnology, #9E10), or GAL4 mouse antiserum (1:1000, Santa Cruz Biotechnology, #sc-510). After extensive washing in TBST, filters were incubated with anti-rabbit horseradish peroxidase-linked

(4) Mutagenesis of the PALB2 WD40 domain identifies variants defective in interaction with BRCA2 and DNA repairJournal of Biological ChemistryDecember 1, 2025Thiago T. Gomes, Anna Beatriz R. Elias, Huaizhi Huang, Chunling Hu, Thales C. Nepomuceno, Giuliana de Gregoriis, Daniel S. Chrispim, Larissa B. Skaf, Fernanda A.R.L. Szpunar, Guilherme Suarez-Kurtz, et al.(Santa Cruz Biotechnology) were incubated with 4 μg of anti-GAL4 antibody (Santa Cruz Biotechnology; sc-510) for 1 h in mild-RIPA buffer. Beads were washed three times with mild-RIPA buffer and then incubated

(5) TANGO2 binds crystallin alpha B and its loss causes desminopathyNat CommunJune 6, 2025Maike Stentenbach, Laetitia A. Hughes, Samuel V. Fagan, Blake Payne, Danielle L. Rudler, Stefan J. Siira, Tim McCubbin, Anaëlle Chopin, Kara L. Perks, Judith A. Ermer, et al.Invitrogen, 1:1000) in Odyssey Blocking Buffer (Li-Cor). GAL4 AD (135398, Abcam, diluted 1:4000), GAL4 DBD (sc 510, Santa cruz, diluted 1:500). IR Dye 800CW Goat Anti-Rabbit IgG or IRDye 680LT Goat Anti-Mouse IgG (Li-Cor)

(6) Subcellular targets and recognition mechanism of Ralstonia solanacearum effector RipE1iScienceMay 16, 2025Dimitra Tsakiri, Konstantinos Kotsaridis, Vassiliki A. Michalopoulou, Ning Zhang, Sotiris Marinos, Nikos Kountourakis, Michael Kokkinidis, Gregory B. Martin, Panagiotis F. SarrisAB_331783 anti-mouse,Cell Signaling Technology,Cat# 7076; RRID: AB_330924 anti-GAL4-TA,Santa Cruz Biotechnology,Cat# sc-510; RRID: AB_627655 anti-GAL4-DBD,Santa Cruz Biotechnology,Cat# sc-1663; RRID: AB_669111 anti-rabbit,Cell Signaling Technology

(7) Both phytochrome A and phyB interact with PHYTOCHROME-INTERACTING FACTORs through an evolutionary conserved phyOPM-APA interactionNat CommunApril 26, 2025Jaehoon Jeong, Yongju Lee, Giltsu Choiα-GST (sc-138, Santa Cruz Biotechnology), α-mCherry (632543, Takara), α-GFP (ab290, Abcam), α-GAL4 DBD (sc-510, Santa Cruz Biotechnology), α-PIF3 (rabbit polyclonal, Abfrontier), and α-TUB (T5168, Sigma). The secondary

(8) Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 axis controls ESC differentiationCellFebruary 20, 2025Giuseppe Quarto, Andrea Li Li Greci, Martin Bizet, Audrey Penning, Irina Primac, Frédéric Murisier, Liliana Garcia-Martinez, Rodrigo L. Borges, Qingzeng Gao, Pradeep K.R. Cingaram, et al.(Cell Signaling, 4909), Flag (Sigma Aldrich, F3165), Myc (Cell Signaling, 2276), GAL4 (Santa Cruz, sc-510), GST (Cell Signaling, 2624). Recombinant protein purification GST and GST-tagged proteins were expressed

(9) The sequence–structure–function relationship of intrinsic ERα disorderNatureJanuary 8, 2025Zhanwen Du, Han Wang, Shuqi Luo, Zixi Yun, Chen Wu, Wangfei Yang, Matthias Buck, Wenwei Zheng, Alexandar L. Hansen, Hung-Ying Kao, et al.catalogue no. sc-374015), β-tubulin (Santa Cruz, catalogue no. sc-166729), Gal4 (Santa Cruz, catalogue no. sc-510), HA-tag (Santa Cruz, catalogue no. sc-7392), His-tag (Millipore Sigma, catalogue no. SAB4301134) and

(10) Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance proteinPLOS PathogensNovember 8, 2024Saet-Byul Kim, Ki-Tae Kim, Solhee In, Namrata Jaiswal, Gir-Won Lee, Seungmee Jung, Abigail Rogers, Libia F. Gómez-Trejo, Sujan Gautam, Matthew Helm, et al.anti-GAL4 (Abcam, Cat No. ab135398, 1:2000 dilution) and anti-GAL4 (DBD) (Santa Cruz Biotechnology, Cat No. sc-510, 1:1000 dilution), respectively. Yeast two-hybrid assay To generate a yeast expression vector with

(11) The N-Terminal Region of the Transcription Factor E2F1 Contains a Novel Transactivation Domain and Recruits General Transcription Factor GTF2H2BiomoleculesOctober 25, 2024Lin Zhao, Rinka Nakajima, Yaxuan Zhou, Mashiro Shirasawa, Mariana Fikriyanti, Yuki Kamiya, Hiroyuki Toh, Hideyuki Komori, Ritsuko Iwanaga, Andrew P. Bradford, et al.Santa Cruz Biotechnology, Danvers, MA, USA, 1:500), anti-β-galactosidase (AHP1292GA, Bio-Rad, 1:500), anti-GAL4 (DBD) (sc-510, Santa Cruz Biotechnology, Danvers, MA, USA, 1:500), anti-GTF2H2 (HPA047001, Merck Sigma-Aldrich, Darmstadt,

(12) LHP1 and INO80 cooperate with ethylene signaling for warm ambient temperature response by activating specific bivalent genesCell ReportsSeptember 24, 2024Zhengyao Shao, Yanan Bai, Enamul Huq, Hong Qiao,Santa Cruz Biotechnology,Cat# sc-46680; RRID: AB_669109 Mouse monoclonal GAL4 [DBD] Antibody,Santa Cruz Biotechnology,Cat# sc-510; RRID: AB_627655 Goat polyclonal anti Mouse Kappa Light Chain,Bio-Rad,Cat#105001; RRID: AB_618653 Goat

(13) Role of the dynamin-related protein 2 family and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thalianaJournal of Cell ScienceApril 15, 2024Nataliia Gnyliukh, Alexander Johnson, Marie-Kristin Nagel, Aline Monzer, David Babić, Annamaria Hlavata, Saqer S. Alotaibi, Erika Isono, Martin Loose, Jiří Frimland anti-HA (GAD) antibodies [anti-GAL4(DBD) (mouse) (RK5C1), monoclonal, Santa Cruz Biotechnology, sc-510, 1:3000; anti-HA (rat) (3F10), monoclonal, Roche, 11867423001, 1:2000]. Protein purification and in vitro

(14) Interaction and assembly of the DNA replication core proteins of Kaposi’s sarcoma-associated herpesvirusMicrobiology SpectrumDecember 12, 2023Lee-Wen Chen, Shie-Shan Wang, Li-Yu Chen, Hsiao-Yun Huang, Si-min He, Chien-Hui Hung, Chun-Liang Lin, Pey-Jium Changincubated with antibodies specific to the FLAG tag (no. A8592; Sigma), GFP (no. G1544; Sigma), GAL4 (sc-510; Santa Cruz), VP16 (sc-7545; Santa Cruz), or actin (no. sc-47778; Santa Cruz). After extensive washing,

(15) The START domain mediates Arabidopsis GLABRA2 dimerization and turnover independently of homeodomain DNA bindingPlant physiologyNovember 28, 2022Mukherjee T, Subedi B, Khosla A, Begler EM, Stephens PM, Warner AL, Lerma-Reyes R, Thompson KA, Gunewardena S, Schrick K.

(16) Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sitesCell host & microbeOctober 12, 2022Iketani S, Hong SJ, Sheng J, Bahari F, Culbertson B, Atanaki FF, Aditham AK, Kratz AF, Luck MI, Tian R, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Mouse monoclonal GAL4 antibody (Clone RK5C1),Santa Cruz Biotechnology,"Catalog #sc-510, RRID: AB_627655" Anti-mouse IgG Kappa light chain binding protein HRP-conjugated,Santa Cruz Biotechnology,"Catalog

(17) Ligand-induced shifts in conformational ensembles that describe transcriptional activationeLifeOctober 12, 2022Khan SH, Braet SM, Koehler SJ, Elacqua E, Anand GS, Okafor CD.using ECL (Thermofisher Scientific, USA) after incubation of mouse monoclonal anti-GAL4DBD antibody (sc510, Santacruz Biotechnology, Santa cruz, USA)) and horse reddish peroxidase linked secondary antibody (sc-525409,

(18) Assessment of small in-frame indels and C-terminal nonsense variants of BRCA1 using a validated functional assayScientific reportsSeptember 28, 2022Nepomuceno TC, Dos Santos APP, Fernandes VC, Elias ABR, Gomes TT, Suarez-Kurtz G, Iversen ES Jr, Couch FJ, Monteiro ANA, Carvalho MA.evaluated by immunoblotting using mouse monoclonal anti-GAL4 DBD (Santa Cruz Biotechnology, catalog number sc-510) and anti-β-actin (Santa Cruz Biotechnology, catalog number sc-47778) as primary antibodies. Horseradish

(19) OCA-T1 and OCA-T2 are coactivators of POU2F3 in the tuft cell lineageNatureJuly 1, 2022Wu XS, He XY, Ipsaro JJ, Huang YH, Preall JB, Ng D, Shue YT, Sage J, Egeblad M, Joshua-Tor L, et al.Sigma-Aldrich, HPA019652, 1:500 or 1:1,000), H3 (Abcam, ab18521, 1:10,000 or 1:50,000), GAL4-DBD (Santa Cruz, SC-510, 1:1,000), HRP-conjugated secondary antibodies (rabbit Cytiva/Amersham, NA934; mouse, Agilent/Dako,

(20) Persister state-directed transitioning and vulnerability in melanomaNature communicationsJune 1, 2022Chauvistré H, Shannan B, Daignault-Mill SM, Ju RJ, Picard D, Egetemaier S, Váraljai R, Gibhardt CS, Sechi A, Kaschani F, et al.Cambridge, UK), KDM5B (NB100-97821, diluted 1:2000, Novus Biologicals, St. Louis, MO, USA) and GAPDH (SC-510, diluted 1:5000, Santa Cruz, Dallas, TX, USA)) were incubated overnight at 4 °C either in PBS containing

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GAL4 [DBD] (RK5C1) Antibody

GAL4 [DBD] (RK5C1) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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GAL4 [DBD] (RK5C1) Antibody from Santa Cruz Biotechnology, Inc.

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