Fig 1: Immunohistochemical staining of BMP15, GDF9, Foxo3a, GAS2, Caspase 3. BMP15 staining in primordial and primer follicles (arrows) were similar in all groups (A1-A4). Oocyte GDF9 staining (stars) score was similar in Cnt and P, and lower than the Ch and F groups (B1-B4). Foxo3a staining in primordial or primer follicles (arrows) were similar in all groups (C1-C4). Oocyte GAS2 staining (stars) intensty were decreased in F and P groups (D3, D4) compared to Cnt and Ch groups (D1, D2). Caspase 3 immunreactivity in follicular cells (arrows) was higher in Ch and F groups (E2, E3) compared to Cnt group (E1). Only representative images were chosen for each protein. Scale bar: 10 μm, IHC, 100x for BMP15, GDF9, Foxo3a and Caspase 3; scale bar: 50 μm, IHC, 40x for GAS2