BrdU (IIB5) Antibody sc-32323 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemBrdU (IIB5) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-32323
Antibody TypePrimary Antibodies
CloneIIB5
Quantity200 µg/ml
ApplicationsIF, IHC(P), FCM
Hostmouse
Conjugate/TagUnconjugated
ClonalityMonoclonal
FormatPurified
TargetBromodeoxyuridine (BrdU)
ReactivityNSR
IsotypeIgG1

Questions and Answers

Can this BrdU antibody be used for flow cytometry applications?

Yes, the BrdU (IIB5) antibody has been shown to work in flow cytometry applications, as evidenced by its use in studies where BrdU incorporation was assessed in various cell types (, ).

What is the recommended protocol for using this antibody in immunofluorescence?

For immunofluorescence, cells should be fixed and permeabilized before incubating with the BrdU antibody. A typical protocol involves denaturing DNA with hydrochloric acid, followed by blocking and overnight incubation with the antibody, as demonstrated in multiple studies (, ).

Is this antibody effective for detecting BrdU incorporation in cancer cell studies?

Yes, the BrdU antibody has been effectively used to detect BrdU incorporation in cancer cell studies, including those examining cell proliferation in various cancer models (, ).

What are some specific applications where this antibody has been cited?

The BrdU antibody has been cited in applications such as immunofluorescence, immunohistochemistry, and flow cytometry, particularly in studies focusing on cell proliferation and DNA replication dynamics (, ).

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Melatonin ameliorates autistic-like behaviors by restoring gut microbiota-derived tryptophan metabolitesCell Mol Life SciMarch 5, 2026Jinghua Shen, Jingjing Gao, Lu Gao, Dongying Yan, Ying Wang, Jia Meng, Hong Li, Dawei Chen, Jie Wu(1:500, Abcam#ab279653), rabbit anti-DCX (1:500, Abcam#ab207175) and mouse anti-BrdU (1:200, Santa Cruz#sc-32323), rabbit anti-Iba1 (1:500, Abcam#ab178846). Then sections were exposed to FITC- or Cy3-conjugated goat

(2) Nuclear effects play an important role in determining codon usage-dependent human gene expressionNat CommunDecember 3, 2025Renu Garg, Pancheng Xie, Jiabin Duan, Huan Liu, Yi Liualiquot of 1/10 volume as input control. Anti-BrU antibody at 2 µg/IP (Santa Cruz Biotechnology, IIB5: sc-32323) and EZview Red Protein G beads (Sigma, E3403) were incubated with RNA for 3 hours at 4 °C, followed

(3) DNA-damage dependent isoform switching modulates RIF1 DNA repair complex assembly and phase separationJournal of Biological ChemistryDecember 1, 2025Adenine Si-Hui Koo, Weiyan Jia, Sang Hwa Kim, Mark Scalf, Claire E. Boos, Yuhong Chen, Demin Wang, Andrew F. Voter, Aditya Bajaj, Lloyd M. Smith, et al.blocked in 3% w/v bovine serum albumin (BSA). Cells were stained with BrdU primary antibody (Santa Cruz, sc-32323) in 3% BSA and incubated overnight at 4 °C, followed by washing in 0.02% v/v PBST (PBS with 0.02% v/v

(4) H263A and SCAN1/H493R mutant TDP1 block TOP1-induced double-strand break repair during gene transcription in quiescent cells and promote cell deathCell Death & DiseaseNovember 28, 2025Diana Rubio-Contreras, Daniel Hidalgo-García, Carmen Angulo-Jiménez, Esperanza Granado-Calle, Margarita Sabio-Bonilla, Jose F. Ruiz, Fernando Gómez-Herrerosovernight. DNA was denatured with 2 N HCl/Triton X-100. Cells were incubated with anti-BrdU (Santa Cruz, sc-32323) at 1:1000 overnight at 4 °C. After that, AlexaFluor-conjugated secondary antibody (Invitrogen) was

(5) Extracellular matrix anchored neutrophils drive pulmonary fibrosis in miceNature CommunicationsNovember 26, 2025Liliang Yang, Piaopiao Sun, Jing Wang, Mengjie Xu, Binyan Zhu, Keqing Yu, Yi Rong, Ruiqing Mao, Shuangfeng Zi, Chi Liu, et al.with BrdU primary antibodies. BrdU incorporation was detected using BrdU antibody (Santa Cruz, Cat# sc-32323, clone IIB5, 1:200), followed by Alexa Fluor–conjugated secondary antibodies (1:2000). Nuclei were counterstained

(6) NeuproGemp, a polyphenol-rich botanical formula, ameliorates Alzheimer’s-like pathology in APP/PS1 mice via inhibition of human glutaminyl cyclaseFrontiers in PharmacologyOctober 21, 2025Tien-Sheng Tseng, Chia-Ching Liaw, Young-Ji Shiao, Ya-I Huang, Yu-Hsiu Cheng, Wang-Chuan Chen, Keng-Chang TsaiThe primary antibodies used included mouse monoclonal anti-BrdU antibody (Santa Cruz Biotechnology, sc-32323) and rabbit polyclonal anti-doublecortin (DCX) antibody (Abcam, ab18723). After washing, sections were

(7) Jab1 regulates HRR mRNA stability to modulate PARP inhibitor sensitivity in triple-negative breast cancerMol CancerAugust 16, 2025Xin Peng, Yingying Wang, Zixiang Yu, Shengfan Huang, Shaolu Zhang, Zhenxing Zhong, Yongzhe Wang, Shanshan Liu, Kailin Wang, Christophe Nicot, et al.(Cell Signaling Technology, 3686 S), p21 (Cell Signaling Technology, 2947 S), β-actin (ABclonal, AC026), BrdU (Santa Cruz, sc-32323), anti-rabbit IgG HRP‐linked antibody (Cell Signaling Technology, 7074 S), anti-mouse IgG HRP‐linked

(8) Mitsugumin 53 drives stem cell differentiation easing intestinal injury and inflammationSignal transduction and targeted therapyJune 11, 2025Pei Y, Fang M, Wu HK, Cui Q, Quan L, Li X, Zhang K, Xie P, Jiang P, Liu Y, et al.anti-PPARα (1:500, Santa Cruz Biotechnology, sc-398394), or anti-BrdU (1:250, Santa Cruz Biotechnology, sc-32323) was at 4 °C overnight and then washed with PBST (0.4% Tween-20). Incubation with anti-rabbit or anti-mouse

(9) Perturbed cell fate decision by schizophrenia-associated AS3MTd2d3 isoform during corticogenesisSci AdvMarch 28, 2025Seunghyun Kim, Youngsik Woo, Dahun Um, Inseop Chun, Su-Jin Noh, Hyeon Ah Ji, Namyoung Jung, Bon Seong Goo, Jin Yeong Yoo, Dong Jin Mun, et al.anti-BrdU rabbit polyclonal (catalog no. PA5-32256, Invitrogen), anti-BrdU mouse monoclonal (catalog no. sc-32323, Santa Cruz Biotechnology), anti–hemagglutinin (HA) rabbit polyclonal (catalog no. A190-108A, RRID:AB_67465,

(10) Regulation of senescence-associated secretory phenotypes in osteoarthritis by cytosolic UDP-GlcNAc retention and O-GlcNAcylationNat CommunFebruary 4, 2025Donghyun Kang, Jeeyeon Lee, Geunho Yook, Sehan Jeong, Jungkwon Shin, Mi-Sung Kim, Yi-Jun Kim, Hyeryeon Jung, Jinsung Ahn, Tae Woo Kim, et al.and 0.1% BSA in PBS for 1 h and incubated with primary antibody against BrdU (clone IIB5, Santa Cruz, sc-32323, 1:200) at 4 °C overnight. The cells were washed three times and incubated with rabbit anti-mouse IgG+IgM

(11) Analysis of a shark reveals ancient, Wnt-dependent, habenular asymmetries in vertebratesNature CommunicationsNovember 25, 2024Maxence Lanoizelet, Léo Michel, Ronan Lagadec, Hélène Mayeur, Lucile Guichard, Valentin Logeux, Dany Séverac, Kyle Martin, Christophe Klopp, Sylvain Marcellini, et al.°C until the desired stages. Following incubation of sections with the anti-BrdU primary antibody (sc-32323, 1/100, Santa Cruz Biotechnology, Dallas, TX, USA), detection was carried out with mouse IgG kappa binding

(12) Galectin-1 Promotes Gastric Carcinoma Progression and Cisplatin Resistance Through the NRP-1/c-JUN/Wee1 PathwayJournal of Gastric CancerJune 13, 2024Zhengyang Pan, Guoxi Xu, Yan Zhang, Meiling Wu, Jiahui Yu, Xujun He, Wei Zhang, Junfeng Hunormal goat serum for 1 hour, the slides were incubated overnight with BrdU primary antibodies (1:50, sc-32323; Santa Cruz Biotechnology, Santa Cruz, CA, USA). The cell nucleus was stained with DNA binding reagent

(13) In Vitro Investigation of Therapy-Induced Senescence and Senescence Escape in Breast Cancer Cells Using Novel Flow Cytometry-Based MethodsCellsMay 15, 2024Tóth F, Moftakhar Z, Sotgia F, Lisanti MP.MA5-14520), BrdU (1:50, IIB5, 200 µg/mL, Santa Cruz Biotechnology, Santa Cruz, CA, USA, cat. number: sc-32323) or γH2AX phospho-S139 (1:500, 9F3, 1 mg/mL, Abcam, Cambridge, UK, cat. number: ab26350) diluted in

(14) Linear interaction between replication and transcription shapes DNA break dynamics at recurrent DNA break ClustersNature CommunicationsApril 27, 2024Lorenzo Corazzi, Vivien S. Ionasz, Sergej Andrejev, Li-Chin Wang, Athanasios Vouzas, Marco Giaisi, Giulia Di Muzio, Boyu Ding, Anna J. M. Marx, Jonas Henkenjohann, et al.the BrdU-incorporated RNA was enriched with the agarose-conjugated anti-BrdU antibody (Santa Cruz, sc-32323, dilution 1:170). At least two technical replicates per experiment condition were performed. GRO-seq

(15) TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcriptionNature communicationsNovember 9, 2023Rubio-Contreras D, Gómez-Herreros F.overnight. DNA was denatured with 2 N HCl/Triton X-100. Cells were incubated with anti-BrdU (Santa Cruz, sc-32323) at 1:1000 overnight at 4 °C. After that, AlexaFluor-conjugated secondary antibody (Invitrogen) was

(16) Global Run-On sequencing to measure nascent transcription in Saccharomyces cerevisiaeMethodsSeptember 1, 2023Michael J. O'Brien, Katherine Gurdziel, Athar AnsarimL of TBST. Anti-BrdU monoclonal antibody was obtained from Santa Cruz Biotechnology (Catalogue # sc-32323). Blocking Buffer contains 0.1% PVP (polyvinylpyrrolidone), 0.1% ultrapure BSA and 100 units SUPERase

(17) The ndrg2 Gene Regulates Hair Cell Morphogenesis and Auditory Function during Zebrafish DevelopmentInternational journal of molecular sciencesJune 11, 2023Wang C, Wang X, Zheng H, Yao J, Xiang Y, Liu D.dilution, Abcam, #ab97959) and a mouse monoclonal anti-BrdU (1:500 dilution, Santa Cruz Biotechnology, #sc-32323, Dallas, TX, USA) were used to label the HCs, supporting cells and proliferative cells, respectively.

(18) Infection-induced extracellular vesicles evoke neuronal transcriptional and epigenetic changesScientific reportsApril 27, 2023Tedford E, Badya NB, Laing C, Asaoka N, Kaneko S, Filippi BM, McConkey GA.immunoprecipitation was performed with protein G Dynabeads and IgG anti-BrdU antibody IIB5 (Santa Cruz Biotechnology, SC-32323). RNA was extracted and RT-qPCR performed as previously described. Methylation sensitive restriction

(19) TERRA stability is regulated by RALY and polyadenylation in a telomere-specific mannerCell reportsApril 25, 2023Savoca V, Rivosecchi J, Gaiatto A, Rossi A, Mosca R, Gialdini I, Zubovic L, Tebaldi T, Macchi P, Cusanelli E.

(20) FAM193A is a positive regulator of p53 activityCell reportsMarch 28, 2023Szwarc MM, Guarnieri AL, Joshi M, Duc HN, Laird MC, Pandey A, Khanal S, Dohm E, Bui AK, Sullivan KD, et al.RRID:AB_10695870 BID,Cell Signaling Technology,Cat#2002; RRID:AB_10692485 BrdU,Santa Cruz Biotechnology,Cat#sc-32323; RRID:AB_626766 Cleaved Caspase-3,Cell Signaling Technology,Cat#9661; RRID:AB_2341188 Caspase-8,Cell Signaling Technology

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BrdU (IIB5) Antibody

BrdU (IIB5) Antibody from Santa Cruz Biotechnology, Inc.

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Questions and Answers

Santa Cruz Biotechnology, Inc.

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BrdU (IIB5) Antibody from Santa Cruz Biotechnology, Inc.

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