Fig 1: Immunohistochemistry of primary tumours engrafted in scid mice and their spontaneous metastases in the lung tissue. a–d IHC staining of representative paraffin-embedded primary tumour tissue and lung tissue with metastasized cells grown in scid mice showing a higher immunoreactivity of L1-CAM, ICAM-1, CD44, and ITGB4 in the tumour cells. Scale bar: 200 and 50 µm. e Images of paraffin-embedded lung sections of scid mice display an immunoreactivity of TSPAN8 within the metastasized cells. This could indicate that these genes are essential for the tumour cells during the emigration process. Arrows label metastasized cells in the lungs. Scale bar: 100 µm