Fig 2: OTUB2 knockdown inhibits tumor proliferation in ESCC cells in vitro. A CCK-8 assay was used to detect cell proliferation; B, C Plate cloning experiment; D, E Cell Cycle Detection by Flow cytometry; F, G Apoptosis was detected by flow cytometry

Fig 3: Bioinformatics analysis of the expression of OTUB2 in esophageal carcinoma and its relationship with YAP1/TAZ (WWTR1). A Expression of OTUB2 in various tumors and corresponding adjacent tissues; B Expression of OTUB2 in esophageal cancer and normal tissues; C The interaction relationship between OTUB2 and YAP1 / TAZ (WWTR1)

Fig 4: OTUB2 regulates YAP1/TAZ in ESCC. A–D Western blotting showing the expression of OTUB2, YAP1, TAZ and CTGF protein; E–G qRT-PCR showing the expression of the OTUB2, YAP1, TAZ and CTGF mRNA

Fig 5: OTUB2 is upregulated in ESCC cell lines. A The expression of OTUB2 in SHEE and KYSE150 detected by immunofluorescence; B Western blotting showing OTUB2 expression in three esophageal squamous cell carcinoma cell lines; C&F Western blotting and qRT-PCR showing lentivirus transfection efficiency; D&E indicate the statistical comparisons of B and C respectively