GOAT ANTI RAT IgG (H/L):HRP 5204-2504 from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemGOAT ANTI RAT IgG (H/L):HRP
CompanyBio-Rad
Price $261.00Supplier Page View Company Product Page
Catalog Number5204-2504
Quantity2 mg
ApplicationsWestern Blotting,ELISA,Immunohistology - Frozen
Conjugate/TagHRP
ReactivityRat
TargetIgG
IsotypeIgG
HostGoat
ImmunogenRat IgG, whole molecule
Domain/Region/Terminus(H/L)
NCBI Full Gene Nameimmunoglobulin heavy constant gamma 1, similar to IG KAPPA CHAIN V-V REGION K2 PRECURSOR
NCBI Gene AliasesRGD1359539, Ighg
ClonalityPolyclonal Antibody

Questions and Answers

Can this goat anti-rat IgG conjugated to HRP be used for Western blotting applications?

Yes, the goat anti-rat IgG conjugated to horseradish peroxidase has been utilized in Western blotting, as evidenced by its application in studies such as those by Nelson et al. (2021) and Sanchez de Groot et al. (2015), where it was employed to detect specific proteins in rat tissue samples.

Is this antibody effective for use in ELISA assays?

The goat anti-rat IgG conjugated to HRP has been shown to work effectively in ELISA assays. For instance, Reynolds et al. (2023) utilized this antibody to quantify antibody titres against specific peptides in their study, demonstrating its suitability for this application.

What is the reactivity of this antibody, and in what species can it be used?

This antibody is specifically reactive to rat IgG, making it suitable for experiments involving rat models. Its specificity is highlighted in various studies, including those that assess immune responses in rat models of disease.

Are there any documented applications of this antibody in immunohistochemistry?

Yes, the goat anti-rat IgG conjugated to HRP has been used in immunohistochemistry, as indicated in the literature. Its application in this context supports its utility for visualizing specific antigens in rat tissue sections, contributing to the understanding of various biological processes.

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Phosphoinositide Depletion and Compensatory β-adrenergic Signaling in Angiotensin II-Induced Heart Disease: Protection Through PTEN InhibitionbioRxivFebruary 27, 2025Taylor L. Voelker, Maartje Westhoff, Silvia G. Del Villar, Phung N. Thai, Nipavan Chiamvimonvat, Madeline Nieves-Cintrón, Eamonn J. Dickson, Rose E. DixonHRP-conjugated secondary antibodies (Bio-Rad/Jackson ImmunoResearch 211-032-171; Biorad 170516; or Biorad 5204-2504; all diluted 1:10,000), washed, and developed using chemiluminescence reagents (Immobilon Classico,

(2) The USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6Nature communicationsOctober 5, 2023Spencer ZT, Ng VH, Benchabane H, Siddiqui GS, Duwadi D, Maines B, Bryant JM, Schwarzkopf A, Yuan K, Kassel SN, et al.were HRP-conjugated goat anti-mouse (1:10000, catalog #STAR207P) and goat anti-rat (1:10000, catalog #5204-2504) from BioRad, and goat anti-guinea pig (1:10000, catalog #6090-05) from SouthernBiotech. Antibodies

(3) Streptococcus pyogenes vaccine candidates do not induce autoimmune responses in a rheumatic heart disease modelnpj VaccinesFebruary 4, 2023Simone Reynolds, Rukshan Ahamed Mohamed Rafeek, Adam Hamlin, Ailin Lepletier, Manisha Pandey, Natkunam Ketheesan, Michael F. Good1:100 to 1:3276800) diluted in 0.5% skim milk in 0.05% PBS Tween-20. After washing, goat anti-rat HRP (5204-2504, Bio-Rad, USA) secondary antibody was applied at 1:10,000 dilution. Plates were developed with o-Phenylenediamine dihydrochloride

(4) Involvement of Ca(V) 2.2 channels and α(2) δ-1 in homeostatic synaptic plasticity in cultured hippocampal neuronsThe Journal of physiologyDecember 1, 2022Pilch KS, Ramgoolam KH, Dolphin AC.secondary Abs at 1:2000 for 1 h (all secondary Abs from Bio‐Rad, raised in goat anti‐rat HRP, cat. no. 5204‐2504, and anti‐mouse HRP, cat. no. 1721011). Protein bands were revealed using ECL reagent (ECL 2, Thermo

(5) Low pH Facilitates Heterodimerization of Mutant Isocitrate Dehydrogenase IDH1-R132H and Promotes Production of 2-HydroxyglutarateBiochemistryJune 29, 2021Sesanto R, Kuehn JF, Barber DL, White KA.with a 1:2000 dilution of anti-rabbit HRP (1706515, Bio-Rad) for the IDH1 antibody or anti-rat HRP (5204-2504, Bio-Rad) for the IDH1-R132H antibody. In all cases, samples were run in technical replicates and negative

(6) The gut microbiome contributes to blood‐brain barrier disruption in spontaneously hypertensive stroke prone ratsThe FASEB JournalJune 21, 2021James W. Nelson, Sharon C. Phillips, Bhanu P. Ganesh, Joseph F. Petrosino, David J. Durgan, Robert M. BryanTween-20 (Bio-Rad) in PBS (VWR). The membrane was then incubated with goat anti-Rat IgG-HRP (Bio-Rad, 5204-2504, 1:10,000) for 1 hour at room temperature. The lower half of the membrane (containing GAPDH) was blocked

(7) Synergistic interactions of PlGF and VEGF contribute to blood-retinal barrier breakdown through canonical NFκB activationExperimental cell researchDecember 15, 2020Lennikov A, Mukwaya A, Fan L, Saddala MS, De Falco S, Huang H.in 1% BSA in PBS-T (Goat anti-Rabbit IgG, 170-6515; Goat anti-Mouse IgG 172-1011; Goat anti-Rat IgG 5204-2504 Bio-Rad) for 1 h at RT. Signals were developed with ECL using a Super Signal West Pico kit (Thermo Fisher

(8) αv-Class integrin binding to fibronectin is solely mediated by RGD and unaffected by an RGE mutationThe Journal of cell biologyDecember 7, 2020Benito-Jardón M, Strohmeyer N, Ortega-Sanchís S, Bharadwaj M, Moser M, Müller DJ, Fässler R, Costell M.horseradish peroxidase (170-6516; Bio-Rad) and goat anti-rat conjugated with horseradish peroxidase (5204-2504; Bio-Rad). To label actin filaments, we used phalloidin labeled with either tetramethylrhodamine B isothiocyanate

(9) The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8The EMBO journalOctober 4, 2019Singh AN, Oehler J, Torrecilla I, Kilgas S, Li S, Vaz B, Guérillon C, Fielden J, Hernandez-Carralero E, Cabrera E, et al.polyclonal)",Sigma‐Aldrich,Cat# A9044; RRID:AB_258431 "Rat IgG, HRP‐conjugated (goat polyclonal)",Bio‐Rad,Cat# 5204‐2504; RRID:AB_619913 "Alexa Fluor 488, Goat Anti‐Mouse IgG (H+L)",Thermo Fisher Scientific,Cat# (A‐11001);

(10) Disruption of the Key Ca²⁺ Binding Site in the Selectivity Filter of Neuronal Voltage-Gated Calcium Channels Inhibits Channel TraffickingCell reportsOctober 1, 2019Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, Zhao P, Patel T, Rothwell SW, Lin P, et al.Hempstead, UK",Cat #1706515; RRID: AB_11125142 Goat anti-rat HRP,"Biorad, Hemel Hempstead, UK",Cat #5204-2504; RRID: AB_619913 Goat anti-mouse HRP,"Biorad, Hemel Hempstead, UK",Cat #1721011; RRID: AB_11125936 Anti-Rat

(11) Mouse TMCO5 is localized to the manchette microtubules involved in vesicle transfer in the elongating spermatidsPLOS ONEAugust 8, 2019Kenya Yamase, Yoko Tanigawa, Yasufumi Yamamoto, Hiromitsu Tanaka, Tohru Komiyadetection, we used 1:10,000diluted horseradish peroxidase-conjugated anti-rat IgG antibody (BioRad, 5204–2504) as a PLOS ONE | https://doi.org/10.1371/journal.pone.0220917 August 8, 2019 3 / 18 Spermatogenesis

(12) The α₂δ-like Protein Cachd1 Increases N-type Calcium Currents and Cell Surface Expression and Competes with α₂δ-1Cell reportsNovember 6, 2018Dahimene S, Page KM, Kadurin I, Ferron L, Ho DY, Powell GT, Pratt WS, Wilson SW, Dolphin AC.RRID:AB_259443 Goat anti-rabbit HRP,Biorad,Cat # 1706515; RRID:AB_11125142 Goat anti-rat HRP,Biorad,Cat # 5204-2504; RRID:AB_619913 Goat anti-mouse HRP,"Biorad,",Cat # 1721011; RRID:AB_11125936 Affinity-purified Cachd1

(13) Tumor necrosis factor‑related apoptosis‑inducing ligand additive with Iodine‑131 of inhibits non‑small cell lung cancer cells through promoting apoptosisOncology LettersMay 4, 2018Ning Yang, Shuzhan Yao, Dong Liuab8226) (all from Abcam, Cambridge, UK). Horseradish peroxidase‑conjugated anti‑rabbit IgG (cat no. 5204‑2504; Bio‑Rad Laboratories, Inc., Hercules, CA, USA) were used at a 1:5,000 dilution and detected using a

(14) Regulatory NLRs Control the RLR-Mediated Type I Interferon and Inflammatory Responses in Human Dendritic CellsFrontiers in immunologyJanuary 1, 2018Fekete T, Bencze D, Szabo A, Csoma E, Biro T, Bacsi A, Pazmandi K.bound antibodies were labeled with anti-mouse (Bio-Rad, Cat. No. 1721011), anti-rat (Bio-Rad, Cat. No. 5204-2504) or anti-rabbit (GE Healthcare, Cat. No. NA934) horseradish peroxidase-conjugated secondary antibodies

(15) Proteome response at the edge of protein aggregationOpen biologyFebruary 1, 2015Sanchez de Groot N, Gomes RA, Villar-Pique A, Babu MM, Coelho AV, Ventura S.a positive control. The antibodies employed were: MCA78G anti-tubulin alpha (Abd Serotec) from rat, 5204–2504 goat antirat (Abd Serotec), A-6455 anti-GFP (Life Technologies) from rabbit, anti-rabbit A9169 (Sigma)

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GOAT ANTI RAT IgG (H/L):HRP

GOAT ANTI RAT IgG (H/L):HRP

GOAT ANTI RAT IgG (H/L):HRP

GOAT ANTI RAT IgG (H/L):HRP

GOAT ANTI RAT IgG (H/L):HRP

GOAT ANTI RAT IgG (H/L):HRP from Bio-Rad (Formerly AbD Serotec)

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GOAT ANTI RAT IgG (H/L):HRP from Bio-Rad (Formerly AbD Serotec)

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