Indirect immunostaining of PFA fixed mouse 3T3 fibroblasts transfected with a Tom70-GFP fusion construct (green) with Guinea pig anti-GFP (dilution 1 : 500; red) and recombinant rabbit anti-α-Tubulin (cat. no. 302 008, dilution 1 : 500; grey). Nuclei have been visualized by DAPI staining (blue).

Indirect immunostaining of PFA fixed brain section from a VGAT-Cre x AAV-floxed-Chann-YFP mouse (GFP in green) and Guinea pig anti-GFP (dilution 1 : 500; red).

Courtesy: Elisa Galliano, PhD, MRC Centre for Developmental Neurobiology, King's College London.

Indirect immunostaining of a PFA fixed cortex section from a transgenic CX3CR1-GFP mouse expressing GFP in microglia (green). GFP has been detected with Guinea pig anti-GFP (cat. no. 132 004, dilution 1 : 500, red). Nuclei have been visualized by DAPI staining (blue).​

Indirect immunostaining of a PFA fixed cortex section from a transgenic CX3CR1-GFP mouse expressing GFP in microglia (green). GFP has been detected with Guinea pig anti-GFP (cat. no. 132 004, dilution 1 : 500, red). Nuclei have been visualized by DAPI staining (blue).​