Mouse anti-Rabbit IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed)
Western Blot: Mouse anti-Rabbit IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) [NBP1-73657] - Mouse anti-Rabbit IgG (H+L) Secondary Antibody [Fluorescein] [NBP1-73657] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Mouse IgG (RABBIT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Mouse anti-Rabbit IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed)
Available conjugates: Available conjugates: PE/Atto594, DyLight 594, DyLight 680, FITC, DyLight 350, HRP, DyLight 650, PE/Cy7, Allophycocyanin/Cy7, Allophycocyanin, Biotin, DyLight 550, PE, DyLight 405, DyLight 755, Janelia Fluor 646Specificity: This antibody was pre-adsorbed against Human, Goat, and Mouse Serum Proteins. No reaction was observed against Human, Goat and Mouse Serum Proteins.Sizes Available: 1 mg