Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed) [NBP1-72944] - Western Blot of Unconjugated Goat anti-Rabbit IgG (H+L) Secondary antibody [DyLight 488] (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Goat anti-Rabbit IgG (H+L) Secondary antibody [DyLight 488] (Pre-adsorbed) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Goat anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed)
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Goat anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed)
Available conjugates: Available conjugates: DyLight 488Specificity: This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.Sizes Available: 0.1 mg