Mouse anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed)
Western Blot: Mouse anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed) [NBP1-72687] - Western Blot of Mouse anti-Rabbit IgG (H+L) Secondary antibody [Alkaline Phosphatase] (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Mouse anti-Rabbit IgG (H+L) Secondary antibody [Alkaline Phosphatase] (Pre-adsorbed) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Mouse IgG (RABBIT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Mouse anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed)
Available conjugates: Available conjugates: PE/Atto594, Alkaline Phosphatase, DyLight 594, DyLight 680, FITC, DyLight 350, HRP, PE/Cy5.5, DyLight 650, PE/Cy7, Allophycocyanin/Cy7, Allophycocyanin, Biotin, DyLight 550, PE, DyLight 405, PerCP, Janelia Fluor 549, DyLight 488, DyLight 755, Janelia Fluor 646Specificity: This antibody was pre-adsorbed against Human, Goat, and Mouse Serum Proteins. No reaction was observed against Goat, Human and Mouse Serum Proteins.Sizes Available: 1 mg