Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 405]

Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 405] [NBP1-72958] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody [DyLight 405]. Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block for 30 min at RT. Primary Antibody: Goat anti-Rabbit IgG Fc Secondary antibody [DyLight 405] 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

Western Blot: Goat anti-Rabbit IgG Fc Secondary Antibody [DyLight 405] [NBP1-72958] - Western Blot of Goat anti-Rabbit IgG Fc Secondary antibody [DyLight 405]. Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block for 30 min at RT. Primary Antibody: Goat anti-Rabbit IgG Fc Secondary antibody [DyLight 405] 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.