Sheep anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed)
Western Blot: Sheep anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed) [NBP1-72678] - Sheep anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] [NBP1-72678] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Sheep anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed)
Available conjugates: Available conjugates: PE/Atto594, Janelia Fluor 549, DyLight 594, DyLight 680, FITC, PE/Cy5.5, PE/Cy7, Allophycocyanin/Cy7, Alkaline Phosphatase, Allophycocyanin, DyLight 550, DyLight 549, DyLight 649, DyLight 488, Texas RedSpecificity: This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.Sizes Available: 1 mg