Western Blot: Sheep anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 549] (Pre-adsorbed) [NBP1-72961] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: 30 min at RT. Primary Antibody: Sheep anti-Rabbit IgG (H+L) Secondary antibody [DyLight 549] (Pre-adsorbed) (Min X Hu, Gt, Ms Serum Proteins) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM . Rabbit F(c) migrates slightly higher.
Sheep anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 549] (Pre-adsorbed)
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Sheep anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 549] (Pre-adsorbed)
Available conjugates: Available conjugates: DyLight 549, AllophycocyaninSpecificity: This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat or Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.Sizes Available: 100 ug