Fig 1: Depletion of Rad52 and Mad2 using the AID system results in loss of viability in diploids.A) Immunoblot and quantification of Rad52 degradation in Rad52-AID homozygous diploids. Cells were treated with 100 nM 5’a-IAA and sampled at the indicated time points. Cdc2 was used as the loading control. B) Control and Rad52-AID strains were spotted onto YES plates with and without 100 nM 5’a-IAA and/or 2 mM HU. Plates were incubated at 32°C for 3 days then imaged. C-D) Immunoblot and quantification of Mad2 degradation in Mad2-AID haploids and Mad2-AID homozygous diploids. Conditions were the same as the Rad52 assay. E) Control and Mad2-AID strains were spotted onto YES plates with and without 100 nM 5’a-IAA and/or 10 μg/mL thiabendazole (TBZ). Plates were incubated at 32°C for 3 days then imaged.
Fig 2: Depletion of Rad52 and Mad2 using the AID system results in loss of viability in diploids. A, b) Immunoblot and quantification of Mad2 degradation in Mad2-AID haploids and Mad2-AID homozygous diploids. Cells were treated with 100 nM 5′a-IAA and sampled at the indicated time points. Cdc2 was used as the loading control. c) Control and Mad2-AID strains were spotted onto YES plates with and without 100 nM 5′a-IAA and/or 10 µg/mL thiabendazole (TBZ). Plates were incubated at 32°C for 3 days and then imaged. d) Immunoblot and quantification of Rad52 degradation in Rad52-AID homozygous diploids. Conditions were the same as the Mad2 assay. e) Control and Rad52-AID strains were spotted onto YES plates with and without 100 nM 5′a-IAA and/or 2 mM HU. Plates were incubated at 32°C for 3 days and then imaged.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for CDC2/CDK1 Antibody (Y100.4) - BSA Free
Available conjugates: Available conjugates: UnconjugatedSizes Available: 0.1 mg (also 0.025 mg)